Authors :
Divya Prasad; Sukriti Suman; Vikash Kumar; Kundan Patel; Gyanendra B. Chand
Volume/Issue :
Volume 9 - 2024, Issue 2 - February
Google Scholar :
http://tinyurl.com/3h52nrku
Scribd :
http://tinyurl.com/r9fsxvnv
DOI :
https://doi.org/10.5281/zenodo.10716045
Abstract :
Arsenic is ubiquitously prevalent metalloid
contaminant released in the aquatic environment as a
consequence of geogenic and anthropogenic activities.
After West Bengal, Bihar has emerged as a new hotspot
for arsenic toxicity in India. Presence of arsenic in
groundwater and soil in various forms has been a matter
of concern for the scientific community globally. The
present study aims to assess the median lethal
concentration (LC50) for sodium arsenite (NaAsO2) in
Danio rerio (zebrafish). Healthy fish were procured from
Ashish Aquarium Traders, Howrah, India, acclimated in
the laboratory for 15 days in ideal laboratory condition
and fed ad libitum. Following a range finding test for
arsenic, a set of doses were administered to the fish for
24h, 48h, 72h and 96h. The LC50 for each assessment
period was separately determined using probit
regression analysis and later confirmed by pilot test.
The24h, 48h, 72h and 96h LC50were determined as
45mg/L, 44mg/L, 41mg/L and 39mg/L respectively. Since
there is great similarity in genome of zebrafish and
humans, the finding of the present acute toxicity study
may be interpreted for the humans.
Keywords :
Acute Toxicity, Anthropogenic, Danio Rerio, Geogenic, LC50, Physic-Chemical, Probit Regression, Sodium Arsenite.
Arsenic is ubiquitously prevalent metalloid
contaminant released in the aquatic environment as a
consequence of geogenic and anthropogenic activities.
After West Bengal, Bihar has emerged as a new hotspot
for arsenic toxicity in India. Presence of arsenic in
groundwater and soil in various forms has been a matter
of concern for the scientific community globally. The
present study aims to assess the median lethal
concentration (LC50) for sodium arsenite (NaAsO2) in
Danio rerio (zebrafish). Healthy fish were procured from
Ashish Aquarium Traders, Howrah, India, acclimated in
the laboratory for 15 days in ideal laboratory condition
and fed ad libitum. Following a range finding test for
arsenic, a set of doses were administered to the fish for
24h, 48h, 72h and 96h. The LC50 for each assessment
period was separately determined using probit
regression analysis and later confirmed by pilot test.
The24h, 48h, 72h and 96h LC50were determined as
45mg/L, 44mg/L, 41mg/L and 39mg/L respectively. Since
there is great similarity in genome of zebrafish and
humans, the finding of the present acute toxicity study
may be interpreted for the humans.
Keywords :
Acute Toxicity, Anthropogenic, Danio Rerio, Geogenic, LC50, Physic-Chemical, Probit Regression, Sodium Arsenite.
