Authors :
Shahid Khurshid
Volume/Issue :
Volume 7 - 2022, Issue 12 - December
Google Scholar :
https://bit.ly/3IIfn9N
Scribd :
https://bit.ly/3GStoD1
DOI :
https://doi.org/10.5281/zenodo.7525159
Abstract :
The CRISPR/Cas9 system has reformed
genome editing and has become extensively offered
through home kits such as the one used in this research.
This study meant to use the CRISPR home kit to make a
genome mutation to the rpsL gene in E. coli DH5a
bacteria, altering the 43rd amino acid from a Lysine to a
Threonine. This alteration would allow the bacteria to
survive on Streptococcic media, which would normally
prevent its growth. The CRISPR home kit used in this
experiment confined all obligatory ingredients, including
Cas9, gRNA, and template DNA, as well as tools for
testing at home
Keywords :
CRISPR Home Kit, Online Stores, Genome Edits, Bacteria, Cas9, GRNA, Template DNA, Genome Mutation, rpsL Gene, Amino Acid, Lysine, Threonine, Strep Media, Growth.
The CRISPR/Cas9 system has reformed
genome editing and has become extensively offered
through home kits such as the one used in this research.
This study meant to use the CRISPR home kit to make a
genome mutation to the rpsL gene in E. coli DH5a
bacteria, altering the 43rd amino acid from a Lysine to a
Threonine. This alteration would allow the bacteria to
survive on Streptococcic media, which would normally
prevent its growth. The CRISPR home kit used in this
experiment confined all obligatory ingredients, including
Cas9, gRNA, and template DNA, as well as tools for
testing at home
Keywords :
CRISPR Home Kit, Online Stores, Genome Edits, Bacteria, Cas9, GRNA, Template DNA, Genome Mutation, rpsL Gene, Amino Acid, Lysine, Threonine, Strep Media, Growth.
