Authors :
Yukti Kanwar; Neena Nair
Volume/Issue :
Volume 7 - 2022, Issue 9 - September
Google Scholar :
https://bit.ly/3IIfn9N
Scribd :
https://bit.ly/3VysD8F
DOI :
https://doi.org/10.5281/zenodo.7223099
Abstract :
Micronutrient zinc is an essential trace
element being crucial for maintaining the normal
biological functions. Pre-pubertal male Wistar rats (35-
45 gm) were divided into 4 groups: (1) Negative Control
(NC) - fed with standard feed (Ashirwad Industries,
Chandigarh) and tap water was provided ad libitum. (2)
Zinc Control (ZC) - Fed with diet containing 100 μg/gm
zinc. Tap water was provided ad libitum. (3) Pair fed
(PF) - Fed with 100 μg/gm Zn diet but the amount of feed
given was equal to the feed consumed by Zinc deficient
group. Tap water was provided ad libitum. (4) Zinc
Deficient (ZD) - fed with 1.00 μg/gm zinc diet and
demineralized water was provided ad libitum.
Experiments were conducted for 2-, 4- and 6- weeks.
Total protein, protein carbonyl, glutathione, superoxide
dismutase (Total SOD, Cu-Zn SOD and MnSOD) and
catalase were assessed. Results revealed significant
(P<0.05) decrease in total protein, glutathione and CuZnSOD activity after dietary zinc deficiency while
concentration of protein carbonyl and activities of total
SOD, MnSOD and catalase increased. Study indicates
that insufficient dietary zinc results in alteration of total
protein, glutathione, SOD and catalase with consequent
formation of protein oxidation (carbonyl) indicating the
generation of reactive oxygen species leading to oxidative
stress which would impede the functional aspects of
brain sub-regions.
Keywords :
Zinc deficiency, Brain sub regions, total protein, protein carbonyl, glutathione, superoxide dismutase and catalase.
Micronutrient zinc is an essential trace
element being crucial for maintaining the normal
biological functions. Pre-pubertal male Wistar rats (35-
45 gm) were divided into 4 groups: (1) Negative Control
(NC) - fed with standard feed (Ashirwad Industries,
Chandigarh) and tap water was provided ad libitum. (2)
Zinc Control (ZC) - Fed with diet containing 100 μg/gm
zinc. Tap water was provided ad libitum. (3) Pair fed
(PF) - Fed with 100 μg/gm Zn diet but the amount of feed
given was equal to the feed consumed by Zinc deficient
group. Tap water was provided ad libitum. (4) Zinc
Deficient (ZD) - fed with 1.00 μg/gm zinc diet and
demineralized water was provided ad libitum.
Experiments were conducted for 2-, 4- and 6- weeks.
Total protein, protein carbonyl, glutathione, superoxide
dismutase (Total SOD, Cu-Zn SOD and MnSOD) and
catalase were assessed. Results revealed significant
(P<0.05) decrease in total protein, glutathione and CuZnSOD activity after dietary zinc deficiency while
concentration of protein carbonyl and activities of total
SOD, MnSOD and catalase increased. Study indicates
that insufficient dietary zinc results in alteration of total
protein, glutathione, SOD and catalase with consequent
formation of protein oxidation (carbonyl) indicating the
generation of reactive oxygen species leading to oxidative
stress which would impede the functional aspects of
brain sub-regions.
Keywords :
Zinc deficiency, Brain sub regions, total protein, protein carbonyl, glutathione, superoxide dismutase and catalase.