GATA 3 immunostainis an important immune histo chemical stain used in surgical pathology for the detection of breast and urothelial carcinomas. In the recently published literature, GATA3 immunostain positivity has been reported in gallbladder adenocarcinoma. This study aims to evaluate GATA3 immunostain positivity in gall bladder adenocarcinoma. Methodology: A cross-sectional observational study was done on 51 biopsy samples diagnosed with gall bladder adenocarcinomain the histopathology department of Chughtai Institute of Pathology.The study was conducted during January 2021-December 2021. Monoclonal antibody GATA3 (LOT P58312121, GeneAb) was applied by manual technique. Results: GATA3 was applied on 51 cases of gall bladder adenocarcinoma with positive external control. The results of our study show negative GATA3immunostain expression in all cases. Conclusion: In our study, no single case showed positivity for GATA3immunostain. Hence, our study supports that GATA3 immunostain positivity can be utilized primarily for detection of tumor of breast, urinary bladder and adnexal origin

Keywords : Gall bladder adenocarcinoma, GATA3immunostain, breast, urothelial carcinoma, pathology