The jaw impression has the potential for crossinfection from blood and saliva attached to the irreversible hydrocolloid (alginate) mold so that infection control is carried out by washing with running water and disinfecting. Disinfection of alginate molds with the spray can use chemical and natural materials. Peronema canescens jack (P. canescens jack) is found in Indonesia, one in West Sumatra, and has antibacterial properties. The sample is tubular with a diameter of 8 mm and a height of 15 mm, consisting of 6 groups, namely the concentration group of 0.5%, 1%, and 2%, as well as the positive control group and negative control group. Microbial test by examining the growth of Staphylococcus aureus (S. aureus) after being sprayed and stored for 10 minutes and then analyzed after 24 hours of incubation with a spectrophotometer. The analysis used the Kruskal-Wallis test and continued with the Mann-Whitney test. There is an effect of spraying Peronema canescence jack at a concentration of 0.5%, which has a more significant growth inhibition than a concentration of 1% and 2%. Disinfection using P. canescens jack can inhibit the growth of S. aureus at a 0.5%

Keywords : Alginate, Disinfectant, Peronema Canescens Jack, Staphylococcus Aureus.