In this study the effect of Sorghum bicolor was
tested on some selected biochemical parameters (direct,
total and indirect billirubin, creatinine, total protein,
serum albumin and urea) was appraised to determine if
the plant extract can reverse the aberrance in the values
of these parameters.
Study design: 40 experimental animals were divvied into
four groups and hemolytic anemia was induced in three
of the groups with two of the groups given the herbal
extract in varying degree (low and high dosage) to assess
the efficacy of the drug on alleviating selected
biochemical parameters, to ultimately investigate the
curative effect of the botanical extract on hemolytic
Place and Duration of Study: Cell and Tissue Culture
Research Laboratory (Drug Discovery Unit),
Department of Biochemistry, Lagos State University
between September 2020 and October 2020.
Methodology: 40 male Wistar rats were divvied into four
equal groups: normal, anemic untreated, anemic low
dosed and anemic high dosed. The low dosed and high
dosed rats were given the extract at 100mg/kg and
500mg/kg respectively. After 7 days of extract
administration, the rats were sacrificed and blood
samples taken though heart puncture were centrifuged
for sera which was assayed using Randox Laboratories
Result: The highest levels of bilirubin, creatinine and
urea level and lowest levels of total protein and albumin
were recorded in the untreated rats, indicating
hemolysis. The treated rats recorded a significant dosedependent reduction in the bilirubin levels and a
significant increase in the total protein levels of the two
treated rats groups.
Conclusion: this study revealed S. bicolor extract to be a
potential amelioration of hemolytic anemia.
Keywords : Hemolytic Anemia, Creatinine, Albumin, Bilirubin, Hemoglobin.