Authors : Indravathi. G

Volume/Issue : Volume 9 - 2024, Issue 11 - November

Google Scholar : https://tinyurl.com/2hckkw4t

Scribd : https://tinyurl.com/3adt2hzd

DOI : https://doi.org/10.5281/zenodo.14332934

Abstract : The physiological, anatomical and morphological features of the plantlets developed under in vitro conditions, have a strong impact on plant survival after transplantation to ex vitro conditions. The leguminous tree, Albizia amara has been selected for tissue culture studies in the present investigation, as it possesses immense medicinal properties. During acclimatization, the effect of bioinoculants on plant physiology was determined in terms of autotrophic activity by measuring the amount of fructose, chlorophyll and carotenoids of the leaf samples. The nutrient content in biotized plants was observed by estimating NPK % and micronutrient content. The water retention capacity of leaves was measured in terms of percentage of water loss. The present study reported that biohardening of tissue cultured plantlets with mixed inoculum of Pseudomonas fluorescens and Trichoderma viride enhanced plant survival rate due to its increased photosynthetic rate, efficient uptake of water, macro and micronutrients.

Keywords : Albizia Amara, Autotrophic Activity, Biohardening, Nutrient Content, Water Retention Capacity.

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