Authors :
O.P Oladipo; S.A Amolegbe; O.G Ademowo; Bamigboye A.Y
Volume/Issue :
Volume 7 - 2022, Issue 12 - December
Google Scholar :
https://bit.ly/3IIfn9N
Scribd :
https://bit.ly/3FTexYl
DOI :
https://doi.org/10.5281/zenodo.7485292
Abstract :
Malaria is endemic and a life-threatening
disease been caused by the bite of a female anopheles
mosquito which destroys the red blood cells. Nano carrier
drug delivery system is of great interest in malaria
research for improving the quality of health care delivery.
This research work focused on development of inorganic
silica nanoparticles as efficient delivery system for
antimalarial drug of Arthemeter (ATM). The mesoporous
silica nanoparticles (MSNPs) both amino modified mobile
crystalline matter (aMCM-41) and mobile crystalline
matter (MCM-41) were synthesized by co-condensation
and sol-gel methods respectively. ATM antimalarial drugs
were loaded in both MCM-41 and aMCM-41 with
chloroform as the solvent under varying effects of time
(1hr, 3hrs and 6hrs), pH (Neutral and Acidic) and
temperature (25oC and 40oC) respectively. The
synthesized nano carrier (MCM-41 and aMCM-41) and
nanodrugs fit well for their expected properties as
depicted from Fourier-Transform infrared spectroscopy
(FT-IR), Nitrogen Physiosorption Isotherm, UV-Visible
Spectroscopy, in vitro kinetic study and in vivo
measurement using P. berghei NK65. The drug loading
capacities (DLC) and Entrapment Efficiency (EE) of the
nano carriers were determined using UV-Visible
spectrophotometry. The FT-IR depicts major functional
groups of the silanol group (Si-OH) and silaxone (Si-O)
which absorbed at 3450 cm-1 and 964 cm-1 respectively for
MCM-41, while after amino functionalization the silanol
group was obstructed. The nanodrugs show only the
functional groups of MSNPs due to the drugs
encapsulation. The synthesized MSNPs (MCM-41 and
aMCM-41) have average pore diameter of 5.1617 nm and
2.9778 nm respectively as expected for the mesoporous
materials which decreases due to adsorption of ATM
encapsulated in MCM-41 and ATM encapsulated in
aMCM-41 to 4.395 nm and 2.5551 nm accordingly. ATM
encapsulates in MCM-41 and aMCM-41: MCM-41Ɔ
ATM and aMCM-41Ɔ ATM have the highest DLC of 79%
and 81% and EE of 65% and 67% respectively which
shows the size effects of MCM-41 compared to aMCM-41.
The in-vitro kinetic studies of the drugs and their
nanodrugs showed that aMCM-41 loaded ATM has the
highest percentage of drugs released compared with
MCM-41. The in-vivo measurement of the combination of
ATM loaded MCM-41 and aMCM-41 shows better bio
performance for plasmodia clearance in infected mice on
the third day compared to the parent drugs. Therefore;
this shows the satisfactory of the synthesized nano carrier
for the delivery of the antimalarial drugs
Keywords :
Antimalarial Drugs (Arthemeter), Delivery System, Mesoporous Silica Nanoparticles (MSNPs), Cocondensation and Sol-gel, Encapsulate.
Malaria is endemic and a life-threatening
disease been caused by the bite of a female anopheles
mosquito which destroys the red blood cells. Nano carrier
drug delivery system is of great interest in malaria
research for improving the quality of health care delivery.
This research work focused on development of inorganic
silica nanoparticles as efficient delivery system for
antimalarial drug of Arthemeter (ATM). The mesoporous
silica nanoparticles (MSNPs) both amino modified mobile
crystalline matter (aMCM-41) and mobile crystalline
matter (MCM-41) were synthesized by co-condensation
and sol-gel methods respectively. ATM antimalarial drugs
were loaded in both MCM-41 and aMCM-41 with
chloroform as the solvent under varying effects of time
(1hr, 3hrs and 6hrs), pH (Neutral and Acidic) and
temperature (25oC and 40oC) respectively. The
synthesized nano carrier (MCM-41 and aMCM-41) and
nanodrugs fit well for their expected properties as
depicted from Fourier-Transform infrared spectroscopy
(FT-IR), Nitrogen Physiosorption Isotherm, UV-Visible
Spectroscopy, in vitro kinetic study and in vivo
measurement using P. berghei NK65. The drug loading
capacities (DLC) and Entrapment Efficiency (EE) of the
nano carriers were determined using UV-Visible
spectrophotometry. The FT-IR depicts major functional
groups of the silanol group (Si-OH) and silaxone (Si-O)
which absorbed at 3450 cm-1 and 964 cm-1 respectively for
MCM-41, while after amino functionalization the silanol
group was obstructed. The nanodrugs show only the
functional groups of MSNPs due to the drugs
encapsulation. The synthesized MSNPs (MCM-41 and
aMCM-41) have average pore diameter of 5.1617 nm and
2.9778 nm respectively as expected for the mesoporous
materials which decreases due to adsorption of ATM
encapsulated in MCM-41 and ATM encapsulated in
aMCM-41 to 4.395 nm and 2.5551 nm accordingly. ATM
encapsulates in MCM-41 and aMCM-41: MCM-41Ɔ
ATM and aMCM-41Ɔ ATM have the highest DLC of 79%
and 81% and EE of 65% and 67% respectively which
shows the size effects of MCM-41 compared to aMCM-41.
The in-vitro kinetic studies of the drugs and their
nanodrugs showed that aMCM-41 loaded ATM has the
highest percentage of drugs released compared with
MCM-41. The in-vivo measurement of the combination of
ATM loaded MCM-41 and aMCM-41 shows better bio
performance for plasmodia clearance in infected mice on
the third day compared to the parent drugs. Therefore;
this shows the satisfactory of the synthesized nano carrier
for the delivery of the antimalarial drugs
Keywords :
Antimalarial Drugs (Arthemeter), Delivery System, Mesoporous Silica Nanoparticles (MSNPs), Cocondensation and Sol-gel, Encapsulate.
