Authors : H. Srutilaya; D. Jayasuriya; Dr. S. Sasikala

Volume/Issue : Volume 11 - 2026, Issue 4 - April

Google Scholar : https://tinyurl.com/mkrzycmw

Scribd : https://tinyurl.com/2dkj8yyx

DOI : https://doi.org/10.38124/ijisrt/26apr160

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Abstract : Dental caries is a biofilm-mediated disease and remains one of the most prevalent non-communicable oral health problems worldwide. Streptococcus mutans plays a key role in the initiation and progression of dental cavities through acid production, enamel demineralization, and biofilm formation. Growing concerns about AMR and the side effects of synthetic agents have raised interest in natural antimicrobial alternatives, such as honey.  Methods: An 86 dental samples (42 plaque + 44 caries) were collected from patients in North Chennai, India. Using standard microbiological and biochemical analysis, the bacterial isolates were identified and confirmed to form biofilm. PCR was performed to detect virulence genes (comE and spaP). The antimicrobial activity of four honey varieties, Marthandam, Orange blossom, Sidr, and Acacia honey, was assessed using agar well diffusion, MIC, and MBC assays. GC–MS analysis was performed to identify bioactive compounds in Marthandam honey.  Results: Among 86 samples analyzed, 59 isolates (68.60%) were identified as Streptococcus mutans. PCR confirmed the presence of virulence genes comE (~384 bp) and spaP (~670 bp). All honey samples showed concentration-dependent antibacterial activity. Marthandam honey demonstrated the strongest inhibition, followed by Orange blossom honey, while Sidr and Acacia honey exhibited moderate activity. GC–MS analysis identified bioactive compounds, including 5- hydroxymethylfurfural (5-HMF).  Conclusion: Marthandam honey demonstrated significant antibiofilm activity against S. mutans, suggesting its potential as a natural antimicrobial agent for the prevention and management of dental cavity.

Keywords : Dental Caries, Streptococcus Mutans, Marthandam Honey, 5-Hydroxymethylfurfural (5-HMF)

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