Authors : Shriya.N; Dr. S. Shobha Rani; Dr. M. Ajitha; Dr. Y. Sridhar Reddy; Sumit Agarwal; Karthik.M; Narendhar.D

Volume/Issue : Volume 9 - 2024, Issue 8 - August

Google Scholar : https://tinyurl.com/yc46654x

Scribd : https://tinyurl.com/2cjv9pxc

DOI : https://doi.org/10.38124/ijisrt/IJISRT24AUG1164

Abstract : A quick, accurate, precise Stability indicating method for Simultaneous estimation of N-Acetylcysteine and Acebrophylline in Tablet dosage form was developed and validated. Chromatogram was run on Waters C18 (150 x 4.6 mm, 2 μm) Column. Mobile phase containing OPA Buffer and Methanol taken in a ratio of 10:90 % v/v was pumped at a flow rate of 1.5 mL/min through column. Cushion utilized in this strategy was OPA. Column temperature was kept at 35°C. Injection volume was 5 μL and Run time was 5 minutes. Sample was scanned at 285 nm. RT’s of N-Acetylcysteine and Acebrophylline were found to be 2.646 min and 2.117 min respectively. The developed method was validated and was found to be Accurate, Precise and Linear over the Concentration Range of 25 % to 175 % of Test concentration. Retention time and Runtime are less and the method was properly validated so this method can be utilized for routine analysis and stability studies of Assay of N-Acetylcysteine and Acebrophylline in Tablet dosage form in industries.

Keywords : N-Acetylcysteine, Acebrophylline, Stability Indicating, Simultaneous Estimation, Tablet Dosage form, RP-HPLC.

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