In-vitro Anti-Urolithiatic Activity of some Yemeni Medicinal Plants Extracts


Authors : Marwa F.M.S. Atef; Abdul Rahman A. Bin Yahya; Adel A.M. Saeed

Volume/Issue : 2nd ICTSA-2022

Google Scholar : https://bit.ly/3TmGbDi

Scribd : https://bit.ly/3TW6Erx

DOI : https://doi.org/10.5281/zenodo.7783844

Kidney stone is one of the most prevalent diseases worldwide. Phytochemicals are responsible for the medicinal activity of plant species. They have the ability for curing various ailment and possesses potential anti-inflammatory, anti-bacterial, anti-oxidant, and antifungal properties. Natural products from medicinal plants, either as pure compounds or as extracted out, provide opportunities for new drug leads because of the unmatched availability of chemical diversity. Due to the rising demand for chemical diversity nowadays in screening programs, seeking therapeutic drugs from herbal products, are quite interesting throughout the world. The present study aims at studying the antiurolithiatic activity of aqueous, methanolic, and hot aqueous extracts of the leaves of Indigofera oblangifolia (Fabaceae), leaves and barks of Capparis Catrilaginea )Capparisaceae( and vegetable collection of Fagonia indica Burm.f. (Zygophyllaceae). First, in-vitro study was conducted to assess anti-urolithiatic effect of plants for all extracts with a standard drug namely Cystone as a control. Turbidity method and calcium oxalate dissolution method were practiced to access the inhibition of stone formation and dissolution of stone crystals respectively. Secondly, extracts were prepared and arranged in different concentrations. The homogenous precipitation method was used to prepare an artificial stone such as calcium oxalate and a semipermeable membrane of eggs was used as dissolution bags. Dissolution models were incubated for 72hrs and after that, the entire content in dissolution bags was estimated spectrophotometrically. The inhibitory activity of extracts on the nucleation of calcium oxalate crystals and the rate of aggregation in calcium oxalate crystals were determined by spectrophotometric assay besides a titration method. Aqueous & alcoholic extracts of Indigofera oblangifolia leaves showed the highest solubility, inhibition, and non-formation of calcium oxalate followed by Capparis Catrilaginea bark, the results were very close to the Cystone control. On the other hand, the lowest effects were for Fagonia indica Burm.f. leaves and Capparis Catrilaginea leaves. Indigofera oblangifolia leaves and Capparis Catrilaginea bark in some extracts exhibited significant in-vitro antiurolithiatic activity. Aqueous and alcoholic extracts of Indigofera oblangifolia leaves and Capparis Catrilaginea bark had similar effects as commonly used Cystone medicine for preventing as well as treating renal stones, even in its crude form. Results guide us for the further detailed investigation and development of new drugs from these medicinal plants.

Keywords : Kidney and Renal Stones, Extracted Plants, Cystone, Anti-Urolithiatic Activity, in-Vitro Study.

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