Authors :
Mohammed Mohammed Abdu Al-Zazaee; Mawhob. M. M. Abdu, Doaa Ahmed Mahmoud; Abdul-Aziz Ahmed Mohammed Abdu
Volume/Issue :
2nd ICTSA-2022
Google Scholar :
https://bit.ly/3TmGbDi
Scribd :
https://bit.ly/3KmZM3m
DOI :
https://doi.org/10.5281/zenodo.7788606
Abstract :
Lipases are class of enzymes catalyzing
aqueous and non-aqueous phases. lipases belong to an
important group of biotechnological enzymes, which
have got industries dairy, , textile, clinical diagnosis,
disease & biomedical importance etc. In this study, we
have isolated an extracellular lipase Bacillus cereus MS6
enzymes from the effluent of a local Sewerage Yemen
Company of ghee & soap industry (YCGSI) City of Taiz.
The lipase production media was optimized of the
enzyme and the purification was performed by a series
of steps as well as using column Cellulose ion exchange
chromatograph, thus SDS- electrophoreses which
indicated a single protein band corresponding to a
molecular weight of approximately 35 kilo-Dalton. The
pH optimum was found to be 9.0 as well temperature at
40 0C, extracellular lipase that we have isolated and
purified in this study has shown thermal and pH
stability and activity in comparable with other bacterial
extracellular lipases.
Keywords :
Bacillus Cereus MS6, Lipase Enzymes, Gel Filtration, Characterization, Lyophilized.
Lipases are class of enzymes catalyzing
aqueous and non-aqueous phases. lipases belong to an
important group of biotechnological enzymes, which
have got industries dairy, , textile, clinical diagnosis,
disease & biomedical importance etc. In this study, we
have isolated an extracellular lipase Bacillus cereus MS6
enzymes from the effluent of a local Sewerage Yemen
Company of ghee & soap industry (YCGSI) City of Taiz.
The lipase production media was optimized of the
enzyme and the purification was performed by a series
of steps as well as using column Cellulose ion exchange
chromatograph, thus SDS- electrophoreses which
indicated a single protein band corresponding to a
molecular weight of approximately 35 kilo-Dalton. The
pH optimum was found to be 9.0 as well temperature at
40 0C, extracellular lipase that we have isolated and
purified in this study has shown thermal and pH
stability and activity in comparable with other bacterial
extracellular lipases.
Keywords :
Bacillus Cereus MS6, Lipase Enzymes, Gel Filtration, Characterization, Lyophilized.
