Snake-Antivenom Activities of Aqueous Extracts of Amaranthus spinosus L. Against Naja subfulva Venom


Authors : Kennedy K. Yego; Eliud N.M. Njagi; George O. Orinda; Joseph K. Gikunju

Volume/Issue : Volume 7 - 2022, Issue 12 - December

Google Scholar : https://bit.ly/3IIfn9N

Scribd : https://bit.ly/3vUvxIW

DOI : https://doi.org/10.5281/zenodo.7527603

Abstract : The present management regime of snake bites requires the use of anti-venom immunoglobulins (Igs). However, these anti-venoms have the limitations of being expensive, requiring cold storage facilities, and having problems of hypersensitivity reactions in some individuals. Amaranthus spinosus plant medicine has traditionally been used in managing snake bites in UasinGishu County, Kenya. However, its efficacy has not been scientifically validated. Therefore, this study aimed to determine in vivo and in vitro the efficacy of the medicinal plant against Naja subfulva venom using the mouse model, agarose-erythrocyte-egg yolk gel plate, and humancitrated plasma methods. The antivenom studies suggest that the aqueous plant extracts possess antivenom activity against N. subfulva venom both in vivo and in vitro. In conclusion, this study confirmed that aqueous extracts of Amaranthus spinosus were effective in neutralizing in vivo and in vitro snake venom activity of Naja subfulva.

Keywords : Median effective dose (ED50), Median lethal dose (LD50), Phospholipase A2, Toxicity, Phytochemicals.

The present management regime of snake bites requires the use of anti-venom immunoglobulins (Igs). However, these anti-venoms have the limitations of being expensive, requiring cold storage facilities, and having problems of hypersensitivity reactions in some individuals. Amaranthus spinosus plant medicine has traditionally been used in managing snake bites in UasinGishu County, Kenya. However, its efficacy has not been scientifically validated. Therefore, this study aimed to determine in vivo and in vitro the efficacy of the medicinal plant against Naja subfulva venom using the mouse model, agarose-erythrocyte-egg yolk gel plate, and humancitrated plasma methods. The antivenom studies suggest that the aqueous plant extracts possess antivenom activity against N. subfulva venom both in vivo and in vitro. In conclusion, this study confirmed that aqueous extracts of Amaranthus spinosus were effective in neutralizing in vivo and in vitro snake venom activity of Naja subfulva.

Keywords : Median effective dose (ED50), Median lethal dose (LD50), Phospholipase A2, Toxicity, Phytochemicals.

Never miss an update from Papermashup

Get notified about the latest tutorials and downloads.

Subscribe by Email

Get alerts directly into your inbox after each post and stay updated.
Subscribe
OR

Subscribe by RSS

Add our RSS to your feedreader to get regular updates from us.
Subscribe