Authors :
Khushbu H. Patel; Ruchi M.Patel; Meghna R. Adhvaryu
Volume/Issue :
Volume 9 - 2024, Issue 5 - May
Google Scholar :
https://tinyurl.com/5xb9x5em
Scribd :
https://tinyurl.com/4vdm6a47
DOI :
https://doi.org/10.38124/ijisrt/IJISRT24MAY998
Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.
Abstract :
Withania somnifera also known as
Ashwagandha used as rasayana (tonic) in Ayurvedic system
of medicine. It is widely considered as Indian Ginseng and
possesses medicinal properties like adaptogenic, antistress, anxiolytic, anti-arthritic, anti-inflammatory etc.
There is long history of microbes having stable symbiotic
relationship with plants aiding them in growth and
survival. This endophytic relationship is also been
observed in medicinal plants. This study is an attempt to
observe the biodiversity of endophytic fungal community
in Withania somnifera and to understand their
colonization. The study was aimed to isolate endophytes
from different organs of Withania somnifera with seasonal
variations. A total of 131 endophytic fungi were isolated
from 450 explants from different organs like leaves, stems
and roots of W. somnifera. The overall colonization rate of
endophytes in winter, summer and monsoon were 42.67%,
7.33% and 37.33% respectively. Among the identified
isolates, most abundant and frequently isolated genera
were Alternaria, Fusarium and Chaetomium sps. The
dominant species isolated in winter were the Fusarium
moniliformae and Chaetomium globosum from leaves with
17.24% and 13.79% dominant frequency, respectively.
While Alternaria raphani and Chaetomium globosum were
most dominant species in summer from leaves with 40%
dominant density. In the monsoon, Microsporum
ferruginem was the most dominant species isolated from
leaves and stem with 61.29% and 42.86%, respectively.
The most recurring endophytic species of Ascomycetes
group were Alternaria alternata and Chaetomium globosum
seen in all seasons.
Keywords :
Endophytic fungi, Withania somnifera, Seasonal Diversity, Colonization frequency.
References :
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Withania somnifera also known as
Ashwagandha used as rasayana (tonic) in Ayurvedic system
of medicine. It is widely considered as Indian Ginseng and
possesses medicinal properties like adaptogenic, antistress, anxiolytic, anti-arthritic, anti-inflammatory etc.
There is long history of microbes having stable symbiotic
relationship with plants aiding them in growth and
survival. This endophytic relationship is also been
observed in medicinal plants. This study is an attempt to
observe the biodiversity of endophytic fungal community
in Withania somnifera and to understand their
colonization. The study was aimed to isolate endophytes
from different organs of Withania somnifera with seasonal
variations. A total of 131 endophytic fungi were isolated
from 450 explants from different organs like leaves, stems
and roots of W. somnifera. The overall colonization rate of
endophytes in winter, summer and monsoon were 42.67%,
7.33% and 37.33% respectively. Among the identified
isolates, most abundant and frequently isolated genera
were Alternaria, Fusarium and Chaetomium sps. The
dominant species isolated in winter were the Fusarium
moniliformae and Chaetomium globosum from leaves with
17.24% and 13.79% dominant frequency, respectively.
While Alternaria raphani and Chaetomium globosum were
most dominant species in summer from leaves with 40%
dominant density. In the monsoon, Microsporum
ferruginem was the most dominant species isolated from
leaves and stem with 61.29% and 42.86%, respectively.
The most recurring endophytic species of Ascomycetes
group were Alternaria alternata and Chaetomium globosum
seen in all seasons.
Keywords :
Endophytic fungi, Withania somnifera, Seasonal Diversity, Colonization frequency.