Screening Tomato Line Resistance to Controlling Sclerotium rolfsii in Cambodia


Authors : Kry Limeang; Kean Sophea; Yem Sokol; Na Sambo; Chan Sovorn; Socheath ONG

Volume/Issue : Volume 8 - 2023, Issue 6 - June

Google Scholar : https://tinyurl.com/253hw2k2

Scribd : https://tinyurl.com/32wjzd5z

DOI : https://doi.org/10.5281/zenodo.10146946

Abstract : Tomatoes are now one of the most commercially important vegetables in Cambodia, where they are the fourth most important agriculture crop and the leading tomato growers. In Cambodia, the climate conditions are favorable for the disease due to high temperatures, humid conditions, and acidic soil, these factors can make the disease survive in the soil for several years in the absence of the plant host. Southern blight on tomato disease, caused by Sclerotium rolfsii, was studied in Cambodia in 2010 and demonstrated that southern stem rot caused the plant lesion on the stem near the soil line, resulting in wilting and the plant dying.Fifty tomato lines, including two local check varieties, were evaluated for disease resistance, and data on growth and yield were measured and analyzed. Apart from this, two screenings had also been done under greenhouse conduction during the dry and wet seasons in order to find the resistant lines. First, the disease was collected, identified, and cultured in the laboratory, and then an amount of 1g of Sclerotium rolfsii was applied directly to the soil on the tomato plant at the age of 30 days. The disease score was evaluated on days 6, 12, and 18 after inoculation. Based on the result of southern blight screening, 4 lines showed a consistent result of being highly disease-resistant (AVTO 1314, AVTO 1715, AVTO 1716, and AVTO 1616). The interactions between the genotypes and growing conditions for all yield traits were significant.

Keywords : Sclerotium rolfsii, resistant variety,Fifty tomato lines and Cambodia variety.

Tomatoes are now one of the most commercially important vegetables in Cambodia, where they are the fourth most important agriculture crop and the leading tomato growers. In Cambodia, the climate conditions are favorable for the disease due to high temperatures, humid conditions, and acidic soil, these factors can make the disease survive in the soil for several years in the absence of the plant host. Southern blight on tomato disease, caused by Sclerotium rolfsii, was studied in Cambodia in 2010 and demonstrated that southern stem rot caused the plant lesion on the stem near the soil line, resulting in wilting and the plant dying.Fifty tomato lines, including two local check varieties, were evaluated for disease resistance, and data on growth and yield were measured and analyzed. Apart from this, two screenings had also been done under greenhouse conduction during the dry and wet seasons in order to find the resistant lines. First, the disease was collected, identified, and cultured in the laboratory, and then an amount of 1g of Sclerotium rolfsii was applied directly to the soil on the tomato plant at the age of 30 days. The disease score was evaluated on days 6, 12, and 18 after inoculation. Based on the result of southern blight screening, 4 lines showed a consistent result of being highly disease-resistant (AVTO 1314, AVTO 1715, AVTO 1716, and AVTO 1616). The interactions between the genotypes and growing conditions for all yield traits were significant.

Keywords : Sclerotium rolfsii, resistant variety,Fifty tomato lines and Cambodia variety.

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