India is one of the most populous country in the world with 2.5 as an average fertility rate. Nevertheless, the trend of fertility rate has been tumbling down in Indian states. Earlier, women were considered to be lone reason for infertility. It took several decades to understand that infertility in males which may either be due to genetic or non-genetic factors. Y-Chromosomal microdeletion is one of the major genetic factor which cause infertility in males. Analysis of Sequence Tagged Sites (STS) markers in Azoospermia Factor regions (AZFa, AZFb, AZFc) at Yq part helps us to know about the deletion frequency in infertile males. Infertile males who adopt Assisted Reproductive Technique (ART) are likely to transmit the infertility to their sons. Molecular diagnosis of Y chromosomal microdeletion is one of the paramount and prognostic techniques to acquire information about the infertility passing from one generation to another. Only little research has been carried out on Y chromosomal microdeletions in Tamilnadu. The aim of our research is to scrutinize the frequency of Y chromosomal microdeletions in infertile males of Coimbatore district, Tamil Nadu. We utilized eight STS primers (sY84, sY86 for AZFa region; sY 99, Sy100, sY 134 for AZFb region; sY 156, sY 254, sY 255 for AZFc region) to detect the microdeletions. Blood samples of thirty infertile men were scrutinized for Y chromosomal microdeletion. Our results showed that the frequency of deletion in AZFa region accounted for 53%; AZFb – 50%; AZFc region- 60% respectively. AZFc region was highly deleted when compared to AZFa and AZFb.
Keywords : Y-Chromosomal Microdeletions, Azoospermic Factor Regions, Sequence Tagged Sites Primer.