This cross sectional study tested the hypothesis -salivary osteocalcin as a noninvasive biomarker of skeletal maturation. Methods: This cross sectional study included 50 subjects [25 males and 25 females] categorized into five CVMS based on lateral cephalographs and 2 ml unstimulated whole saliva samples taken from each patient on the same day. Salivary osteocalcin activity in unstimulated whole saliva were compared among five CVMS.