Authors :
Tukur Zayyanu; Karnawat Monika
Volume/Issue :
Volume 8 - 2023, Issue 10 - October
Google Scholar :
https://tinyurl.com/97e9tmfn
Scribd :
https://tinyurl.com/457eeptn
DOI :
https://doi.org/10.5281/zenodo.10020473
Abstract :
Background:
The distinguishing features of neurodegenerative
disorders include the death of neurons in the brain or
spinal cord that, over time, results in the loss of a
particular neuronal subtype or indiscriminate loss of
neuronal populations, consequently Alzheimer’s and
Huntington's diseases are said to cause the loss of
neurons, while Parkinson's disease specifically and
insufficiently damages dopaminergic neurons in the
substantia nigra..Aims:
In this study, inducers are used inside in vitro
examinations of neuroblastoma cells as well as a fresh,
contemporary nanobiotechnological approach to disease
therapy.Method:
The materials' cytotoxicity was evaluated using the
MTT colorimetric test technique with minor
modifications.The
concentration ranges for the test samples were 160, 120,
80, 40, 200, μg/ml were added to a confluent monolayer
plate containing the cell lines that had been cultured for
24 hours. The supernatant was collected after 72 hours
of incubation at 37°C in a 5% CO2 incubator, and 25 μl
of MTT reagent (2 mg/ml) was added to each well.Result:
The cell lines SH-S5Y5. After 24 hours of
treatment, cells were suspended and adherent,
undifferentiated neuroblastoma cells, while cells after
being induced with IC50 concentration of
glyceraldehyde, Blebbing cells are a sign of early
apoptosis. Cell after dopamine IC50 concentration
induction. very few circularized cells. Cells were
beginning to differentiate, Cell after treatment with IC50
concentrations of dopamine and Encapsulation Nano
Curcumin. Absence of any circularized cell indicating all
cells are adhered.Conclusion:
This work highlighted the possibility that
Encapsulated Nano Curcumin might be a potent
therapeutic agent, especially for the treatment ofneurodegenerative diseases like Parkinson's Disease.
However, more pre-clinical and clinical research is still
required.
Keywords :
Encapsulated Nano Curcumi, Neurodegenerative diseases and Sh-Sy-5y Cell Line.
Background:
The distinguishing features of neurodegenerative
disorders include the death of neurons in the brain or
spinal cord that, over time, results in the loss of a
particular neuronal subtype or indiscriminate loss of
neuronal populations, consequently Alzheimer’s and
Huntington's diseases are said to cause the loss of
neurons, while Parkinson's disease specifically and
insufficiently damages dopaminergic neurons in the
substantia nigra..Aims:
In this study, inducers are used inside in vitro
examinations of neuroblastoma cells as well as a fresh,
contemporary nanobiotechnological approach to disease
therapy.Method:
The materials' cytotoxicity was evaluated using the
MTT colorimetric test technique with minor
modifications.The
concentration ranges for the test samples were 160, 120,
80, 40, 200, μg/ml were added to a confluent monolayer
plate containing the cell lines that had been cultured for
24 hours. The supernatant was collected after 72 hours
of incubation at 37°C in a 5% CO2 incubator, and 25 μl
of MTT reagent (2 mg/ml) was added to each well.Result:
The cell lines SH-S5Y5. After 24 hours of
treatment, cells were suspended and adherent,
undifferentiated neuroblastoma cells, while cells after
being induced with IC50 concentration of
glyceraldehyde, Blebbing cells are a sign of early
apoptosis. Cell after dopamine IC50 concentration
induction. very few circularized cells. Cells were
beginning to differentiate, Cell after treatment with IC50
concentrations of dopamine and Encapsulation Nano
Curcumin. Absence of any circularized cell indicating all
cells are adhered.Conclusion:
This work highlighted the possibility that
Encapsulated Nano Curcumin might be a potent
therapeutic agent, especially for the treatment ofneurodegenerative diseases like Parkinson's Disease.
However, more pre-clinical and clinical research is still
required.
Keywords :
Encapsulated Nano Curcumi, Neurodegenerative diseases and Sh-Sy-5y Cell Line.
