Authors : Amith B; Harshitha K. N.; Vijay R; Yogeesh A. S.; Shivandappa; Narendra Kumar S.

Volume/Issue : Volume 10 - 2025, Issue 2 - February

Google Scholar : https://tinyurl.com/ymfpsk86

Scribd : https://tinyurl.com/4vthxner

DOI : https://doi.org/10.5281/zenodo.14908897

Abstract : Background: Microbial diversity is what decides the flavor, aroma, and quality of beer. These microbes include bacteria and yeast. These microorganisms can cause spoilage when their population grows unchecked and fermentation when in check. Current traditional techniques to identify the microbial flora have poor resolution and scale up very badly. High-resolution sequencing technologies, such as nanopore sequencing, offer a great look into microbial communities, making their role in the brewing process considerably clearer. Bioinformatics platforms, such as Galaxy.org, offer user-friendly tools for processing and analyzing complex sequencing data, providing advanced microbial characterization capability to researchers and industry professionals  Objectives: This study aimed to isolate and characterize microbial communities in beer using advanced nanopore sequencing technologies combined with the Galaxy.org platform for bioinformatics analysis. Specifically, the objectives were to:  Extract DNA from beer samples and generate long-read sequencing data through the Oxford Nanopore MinION platform.  Use Galaxy.org to process and analyze data: base calling, quality control, taxonomic classification, and visualization.  Identify key microbes, such as bacteria and yeast, emphasizing species linked to fermentation and spoilage.  Demonstrate nanopore sequencing and Galaxy.org as a powerful, user-friendly tool for the identification of microorganisms in brewing, helping quality control and optimization of processes.  Explain how the methodology has wider applications in food and beverage microbiology

Keywords : Nanopore Sequencing, Galaxy.Org, Beer Microbiology, Microbial Identification, Brewing, Bioinformatics.

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