Authors :
Dr. D.P. Sivasakti Balan; V. Santhanalakshmi; R. Fiza; V. Akshita; J.D. Krishna; Dharshan Raj
Volume/Issue :
Volume 9 - 2024, Issue 11 - November
Google Scholar :
https://tinyurl.com/49445ta5
Scribd :
https://tinyurl.com/2vzvamhm
DOI :
https://doi.org/10.38124/ijisrt/IJISRT24NOV1314
Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.
Abstract :
This study investigates the Phytonutrient such
as Xanthophyll,Carotenoid and Chlorophyll b in
Erythrina variegata and Sesbania grandiflora under varies
conditions.which are commonly consumed in tropical and
subtropical regions for their health benefits. Our primary
aim was to assess the loss of chlorophyll pigments during
boiling and shade drying processes. Thin Layer
Chromatography (TLC) using silicon-coated sheets was
employed as an analytical tool to detect and quantify the
pigments in the samples. The Rf (Retention Factor) values
for various pigments were calculated and recorded to
derive the results.
Our findings indicate that temperature-sensitive
pigments like xanthophylls degrade when the leaves are
boiled, while shade-dried leaves showed a reduction in
chlorophyll pigments (Chlorophyll a and Chlorophyll b)
but retained xanthophylls. Additionally, ethanol was used
as a solvent to extract pigments, revealing the presence of
carotene in Sesbania grandiflora leaves. The study
highlights the importance of optimizing cooking and
preservation methods to retain phytonutrients, thereby
maximizing the therapeutic and nutritional benefits of
plant-based foods. Future research could focus on
refining these methods to reduce nutrient loss,
contributing to a more holistic approach to diet and
health.
Keywords :
Phytonutrient, Xanthophyll, Carotenoid, Chlorophyll b, Erythrina variegata and Sesbania grandiflora, TLC, Temperature.
References :
- Agricultural Research Service, United States Department of Agriculture. (2017, December 18). Erythrina variegata. Germplasm Resources Information Network. https://www.ars-grin.gov
- Bureau of Plant Industry, Department of Agriculture, Republic of the Philippines. (2019, November 1). Katuray Production Guide (PDF). Archived from the original (PDF) on February 9, 2021.
- Chopra, R. N., Nayar, S. L., & Chopra, I. C. (1956). Glossary of Indian Medicinal Plants. Council of Scientific and Industrial Research.
- Das, B., & Mandal, S. (2020). Chlorophyll and chlorophyllin as modifiers of genotoxic effects. Nutrition and Cancer, 72(5), 783-792. https://doi.org/10.1080/01635581.2015.990573
- Dharmalingam, S., et al. (2018). Photosynthetic pigment composition and lightharvesting efficiency in Erythrina variegata. Journal of Plant Biology, 61(3), 231–239.
- Duke, J. A., & Ayensu, E. S. (1985). Medicinal Plants of China. Reference Publications.
- Ferruzzi, M. G., et al. (2015). The fate of chlorophyll within the human gastrointestinal tract: Research implications for food science and nutrition. Journal of Nutrition, 145(10), 1981–1988.
- Ferruzzi, M. G., & Blakeslee, J. (2007). Digestion, absorption, and cancer-preventive activity of dietary chlorophyll derivatives. Nutrition Research, 27(1), 1-12.
- Gruszecki, W., et al. (1999). Xanthophyll pigments in light-harvesting complex II in monomolecular layers: Localization, energy transfer, and orientation. Biochimica et Biophysica Acta, 1415(1), 85-92. https://doi.org/10.1016/S0005-2728(99)00052-1
- Huxley, A. (Ed.). (1992). New RHS Dictionary of Gardening. Macmillan. ISBN 0-33347494-5
- Islam, M. T., et al. (2020). Sesbania grandiflora: A review on its phytochemistry and pharmacology. Journal of Ethnopharmacology, 248, 112333.
- MacKeen, D. (2021, July 2). Influencers are drinking chlorophyll water. But why? The New York Times.
- Manikandan, P., & Prabhakaran, R. (2015). Chlorophyll content and photosynthetic efficiency of selected medicinal plants. Journal of Plant Sciences, 10(3), 124-130.
- Muneer, S., Kim, E. J., Park, J. S., & Lee, J. H. (2014). Influence of green, red, and blue light-emitting diodes on multiprotein complex proteins and photosynthetic activity under different light intensities in lettuce leaves (Lactuca sativa L.). International Journal of Molecular Sciences, 15(3), 4657–4670. https://doi.org/10.3390/ijms15034657
- Nagini, S., Palitti, F., & Natarajan, A. T. (2015). Chemopreventive potential of chlorophyllin: A review of the mechanisms of action and molecular targets. Nutrition and Cancer, 67(2), 203–211. https://doi.org/10.1080/01635581.2015.990573 • Pa’ageorgiou, G. C., & Govindjee, A. (2009). Viewpoint: Why chlorophyll a? Photosynthesis Research, 99, 85–98. https://doi.org/10.1007/s11120-008-9395-x
- Taiz, L., Zeiger, E., Møller, I. M., & Murphy, A. (2015). Plant Physiology and Development (6th ed.). Sinauer Associates.
- Tiwari, M., et al. (2021). Chlorophyll and its derivatives in plant systems: Their role in photosynthesis and potential applications. Frontiers in Plant Science, 12, 620997. https://doi.org/10.3389/fpls.2021.620997
- Virtanen, O., Constantinidou, E., & Tyystjarvi, E. (2022). Chlorophyll does not reflect green light: How to correct a misconception. Journal of Biological Education, 56(5), 552–559. https://doi.org/10.1080/00219266.2020.1858930
This study investigates the Phytonutrient such
as Xanthophyll,Carotenoid and Chlorophyll b in
Erythrina variegata and Sesbania grandiflora under varies
conditions.which are commonly consumed in tropical and
subtropical regions for their health benefits. Our primary
aim was to assess the loss of chlorophyll pigments during
boiling and shade drying processes. Thin Layer
Chromatography (TLC) using silicon-coated sheets was
employed as an analytical tool to detect and quantify the
pigments in the samples. The Rf (Retention Factor) values
for various pigments were calculated and recorded to
derive the results.
Our findings indicate that temperature-sensitive
pigments like xanthophylls degrade when the leaves are
boiled, while shade-dried leaves showed a reduction in
chlorophyll pigments (Chlorophyll a and Chlorophyll b)
but retained xanthophylls. Additionally, ethanol was used
as a solvent to extract pigments, revealing the presence of
carotene in Sesbania grandiflora leaves. The study
highlights the importance of optimizing cooking and
preservation methods to retain phytonutrients, thereby
maximizing the therapeutic and nutritional benefits of
plant-based foods. Future research could focus on
refining these methods to reduce nutrient loss,
contributing to a more holistic approach to diet and
health.
Keywords :
Phytonutrient, Xanthophyll, Carotenoid, Chlorophyll b, Erythrina variegata and Sesbania grandiflora, TLC, Temperature.