- In the present investigations the effect of
growth regulators for callus initiation was studied in
India’s important Pulse crop Cicer arietinum to observe
its response. Six genetic lives of Cicer arietinum were
screened for studying morphogenic potential. The intact
seedlings were regenerated and develop into shoots from
cotyledonary-node on B1 basal medium. However, there
was variation in response from different cultivars.
Experimental study proved that excellent growth in
cytokinin supplemented with benzylaminopurine. The
response of regeneration was observed good in
combination of cytokinin and benzylaminopurine. When
cotyledonary node were isolated, the number of multiple
shoots did not increase than on intact seedlings. There
was little swelling observed in different explants like
roots, hypocotyl and epicotyl.
Keywords : Cicer arietinum, auscius, cytokinin, cotyledon.