In the last two decades, increase in multidrug resistance (MDR) of nosocomial bacterial has become a major health challenge worldwide. Enterobacteriaceae are significant causes of serious infections, and many of the clinical important members of this family are becoming increasingly resistant to currently available antibiotics. In this study, one hundred and thirteen nonrepeated multidrug resistant enterobacteriaceae were collected from a tertiary hospital for the study and screened for betalactamase production using penicillinphenol red reagent. E. coli (38), K. Pneumonae (20), Shigellasp (24) and Salmonella sp (31) were the organisms of interest. Results showed the isolates had a MARI of 0.6 and 48.67% of isolates were betalactamase producers. Isolates were observed to be resistant to various antibiotics with Augmentin having the highest mean resistance rate of 0.98, Ampiclox 0.84, Perfloxacillin 0.82, Erythromycin 0.81, Zinacef 0.79, Amoxicillin and Ofloxacillin 0.77, Septrin and Rocephin 0.75, Chloramphenicol 0.67, Ciprofloxacillin 0.59, Gentamycin 0.56 and streptomycin 0.44. Multi drug resistance in isolates were very high with isolates been resistant to at least six antibiotics and this is linked with the production of betalactamase which render betalactam antibiotics infective. There is need for closer monitoring of drug resistant trends and betalactamase production in both clinical and community settings. This will go a long way in reducing the spread of antibiotic resistance.