Klebisella pneumoniae and Escherichia coli
are the major extended spectrum beta-lactamases
(ESBLs) producing microorganisms and are responsible
for failures in treating urinary tract infections (UTIs)
because of its resistance to antibiotics. This study aimed
at evaluating the prevalence and antibiogram of ESBLs
producing E. coli and Klebsiella pneumoniae isolated
from the urine of child-bearing women. A total of eighty
(80) urine samples were collected from both pregnant
and non-pregnant women within the age ranges of 15 –
44 years. The samples were cultured on CLED agar for
the detection of the organism and identified by other
standard microbiological methods. Phenotypic screening
test in the presence of ceftazidime (30 μg), cefotaxime (30
μg), and ceftriaxone (30 μg) were used to detect the
ESBLs producing organisms. The double disc synergy
test (DDT) was used to confirm the ESBL- producing
organisms. A disc of amoxicillin + clavulanic acid
(20/10μg) was placed at the centre of the Mueller Hilton
agar plate and ceftazidime (30 μg), cefotaxime (30 μg),
and ceftriaxone (30 μg) were placed at a distance of 20
mm from the Amoxicillin-clavuanic acid disc. Enhanced
inhibition zone of any of the discs on the site facing the
amoxicillin + clavulanic acid disc was considered as
ESBLs producer. The ESBL producers were found more
on age range of 26-30 (42.5%). The prevalence rate of
ESBL producing organisms were detected in 61.54%
(n=70) of pregnant women and 38.46% (n=10) of nonpregnant women (p<0.05). Escherichia coli accounted for
28.75% (n=23) of the ESBL producing bacteria while
Klebsiella pneumoniae accounted for 3.75% (n=3).
ESBLs producing E. coli and Klebsiella pneumoniae
showed high resistance to cefotaxime (100%), and
ceftriaxone (100%) and completely sensitive to Amikacin
(100%) and Iminipen (100%). From this study the
antibiotic resistance pattern associated with ESBLs
producing E.coli and Klebsiella pneumoniae is a useful
guide for treatment and management of infections
caused by ESBLs producing bacteria.
Keywords :
Extended Spectrum Beta-Lactamases (Esbls). Antibiotic Resistant, Phenotypic Screening, Child-Bearing Age Women, Multidrug Resistance
