Authors :
Randriamamisolonirina Tendrinarisoa; Ravoavy Hajarivelo; Andriantsimahavandy Abel; Maminiaina Olivier Fridolin
Volume/Issue :
Volume 7 - 2022, Issue 4 - April
Google Scholar :
https://bit.ly/3IIfn9N
Scribd :
https://bit.ly/3kZfc0j
DOI :
https://doi.org/10.5281/zenodo.6511818
Abstract :
Newcastle disease (ND) is one of the avian
viruses that cause considerable economic losses
worldwide. It is caused by a virus called Newcastle
disease virus (NDV). Vaccines were available to reduce
the devastating effect of ND. However, outbreaks of the
disease were still found, even in vaccinated flocks. In
order to generate a new multi-epitopic vaccine, this study
aims to predict all conserved epitopes in the
hemagglutinin neuraminidase (HN) of NDV isolated in
Madagascar between 1992 and 2011; using bioinformatics
tools. A total of 12 HN protein sequences were extracted
from the National Center of Biotechnology Information
(NCBI) database. To know the conserved regions, they
were aligned in BIOEDIT 7.2.5. Then, immunoinformatics servers were used to predict the B and T-cells
epitopes present in all sequences. The predicted T-cells
epitopes are further docked with two chickens Major
Histocompatibility Complex (MHC) to find out their
interaction capacity. As results, we identified 39
conserved epitopes including 17 B-cells epitopes and 22 Tcells epitopes. The majority of these epitopes are localized
at the globular head of HN, forming 11 antigenic sites.
Some of them are already identified by other researchers
while some others are newly identified in this study.
Nevertheless, all of these epitopes could be used in the
design of a new vaccine against ND. Since this is a
preliminary and in silico study, further study is needed to
valorize these results.
Keywords :
Newcastle disease; NDV; Hemagglutinin Neuraminidase; epitopes; Bioinformatics; immunoinformatics.
Newcastle disease (ND) is one of the avian
viruses that cause considerable economic losses
worldwide. It is caused by a virus called Newcastle
disease virus (NDV). Vaccines were available to reduce
the devastating effect of ND. However, outbreaks of the
disease were still found, even in vaccinated flocks. In
order to generate a new multi-epitopic vaccine, this study
aims to predict all conserved epitopes in the
hemagglutinin neuraminidase (HN) of NDV isolated in
Madagascar between 1992 and 2011; using bioinformatics
tools. A total of 12 HN protein sequences were extracted
from the National Center of Biotechnology Information
(NCBI) database. To know the conserved regions, they
were aligned in BIOEDIT 7.2.5. Then, immunoinformatics servers were used to predict the B and T-cells
epitopes present in all sequences. The predicted T-cells
epitopes are further docked with two chickens Major
Histocompatibility Complex (MHC) to find out their
interaction capacity. As results, we identified 39
conserved epitopes including 17 B-cells epitopes and 22 Tcells epitopes. The majority of these epitopes are localized
at the globular head of HN, forming 11 antigenic sites.
Some of them are already identified by other researchers
while some others are newly identified in this study.
Nevertheless, all of these epitopes could be used in the
design of a new vaccine against ND. Since this is a
preliminary and in silico study, further study is needed to
valorize these results.
Keywords :
Newcastle disease; NDV; Hemagglutinin Neuraminidase; epitopes; Bioinformatics; immunoinformatics.
