Authors : M. Nur Salim; Siti Aisyah; Dian Masyitha; Rosmaidar; Rumi Sahara Zamzami; Nurlena Andalia; Chairunnisa Salsabila Simagunsong

Volume/Issue : Volume 9 - 2024, Issue 8 - August

Google Scholar : https://tinyurl.com/578zv8j6

Scribd : https://tinyurl.com/yck8jame

DOI : https://doi.org/10.38124/ijisrt/IJISRT24AUG1115

Abstract : Incision wounds are narrow, elongated wounds caused by sharp objects. One of the factors that hinders wound healing is high blood sugar levels in the condition of diabetes mellitus. This study aims to determine the effectiveness of Muntingia calabura L leaf extract cream in accelerating the healing process of incision wounds during the remodeling period on the skin of white rats with diabetes mellitus. The experimental animals used were 12 male white rats (Rattus norvegicus) with an average weight of 150-200 grams and were 2-3 months old. The rats were divided into 4 treatment groups with 3 repetitions. The K1 group as a negative control is given a cream base. The K2 group as a positive control was given 0.1% silver sulfadiazine cream and metformin. The K3 group was given a 5% Muntingia calabura L leaf extract cream and an oral extract of Muntingia calabura L leaves. The K4 group was given a 15% Muntingia calabura L leaf extract cream and an oral extract of Muntingia calabura L leaves. All treatments were carried out for 14 days. The results of the statistical test on the average number of fibroblast cells showed a real difference (P<0.05) between the four groups. The results of the statistical test on the mean collagen density score in the K3 and K4 treatment groups showed a real difference (P<0.05) compared to the K1 and K2 control groups. It can be concluded that the administration of 5% Muntingia calabura L leaf extract cream can reduce the number of fibroblast cells and increase collagen density, thereby accelerating the healing of incision wounds during the remodeling phase on the skin of white rats with diabetes mellitus.

Keywords : Muntingia Calabura L Leaves; Incision Wounds; Fibroblast Cells; Collagen Fibers.

References :

1. Andalia, N., Salim, M. N. and Balqis, U. (2021). Decreasing blood glucose levels using Muntingia calabura L. leaf extract in rats with diabetes mellitus. Advances in Biological Sciences Research. 12:179-183.
2. Binawati, D. K. and Amilah, S. (2013). Effect of cherry leaf (Muntinga calabora) bioinsecticides extract towards mortality of worm soil (Agrotis ipsilon) and armyworm (Psodoptera exiqua) on plant leek (Allium fistolum). WAHANA, 61(2):51-57.
3. Buraerah, H., Abdullah, A. Z. and Hanis, M. (2010). Analysis of risk factors for type 2 diabetes mellitus at Tanrutedong Health Center, Sidennreg Rappan. National Scientific Journal, 35(4):228.
4. Domenico, D. E. G. (2020). Silver sulfadiazine eradicates antibiotic-tolerant Staphylococcus aureus and Pseudomonas aeruginosa biofilms in patients with infected diabetic foot ulcers. Journal of Clinical Medicine, 9(12):3807.
5. Febram, B., Wientarsih, I. and Pontjo, B. (2010). Activity of ointment preparations of ambon banana tree stem extract (Musa paradisiaca var sapientum) in the process of wound healing in mice (Mus musculus albinus). Journal of Traditional Medicine, 15(3):121-137.
6. Fitri, N. and Wael, S. (2015). The use of stem and leaf extract cream (Peperomia pellucida L.H.B.K) in the healing process of burns in white rats (Rattus norvegicus). Molluca Medica, 8(1):82-97.
7. Frisca, Sardjono, C. T. and Sandra, F. (2009). Angiogenesis: pathophysiology and clinical application. Maranantha Medical Journal, 8(2):174-187.
8. Giri, I. M. D. S., Wardani, I. G. A. A. K. and Suena, N. M. D. S. (2021). Secondary metabolic role of plants in collagen formation in the skin of mice with burns. USADHA:Journal of Traditional Medicine Integration. 1(1):23-29.
9. Gonzalez, A. C. O., Andrade, Z. A., Costa, T. F. and Medrado, A. R. A. P. (2016). Wound healing – a literature review. An Bras Dermatol, 91(5): 614-620.
10. Gunawan, S. A., Berata, I. K. and Wirata, I. W. (2019). Skin histopathology in the healing of incision wounds in white rats after administration  of extracellular matrix (ECM) derived from porcine vesica urinaria. Indonesia Medicus Veterinus, 8(3):313-324.
11. Harris, A., Amran, C. M. F., Salim, M. N., Balqis, U., Armansyah, T., Karmil, T. F. and Riady, G. (2019). Efficiacy of Jatropha cream (Jatropha curcas L.) on maturation phase of cutaneus healing process in mice (Mus musculus). Jurnal Medika Veterinaria, 13(2):274-280.
12. Hasliani. (2021). Integument System. CV. Tohar Media, Makassar.
13. Haviz, M. (2012). Insulin shock and its relationship with the body's metabolism. Journal of Science, 4(2):185-191.
14. Iryani., Iswendi. and Katrina, I. T. (2017). Anti-diabetes mellitus activity test of secondary metabolite compounds of water fraction from black glutinous rice (Oryza satival. Var glutinosa) in white mice. Exact Facts, 18(1):54-60.
15. Kalangi, S. J. R. (2013). Skin histophysiology. Journal of Biomedicine (JBM), 5(3):12-20.
16. Kanzaki, T., Morisaki, N., Shiina, R. and Saito, Y. (1998). Role of transforming growth factor-β pathway in the mechanism of wound healing by saponin from Ginseng Radix rubra. Brit. J. Pharmacol, 125:225-262.
17. Kemp, R.W. (2000). Housing and maintenance Handling and Restraint. (The Handbook of Experimental Animals). NCB, London.
18. Krinke, G. (2000). The Laboratory Rat. Academic Press, San Diego.
19. Kurniati, M. F. and Alfaqih, M. R. (2022). The relationship between age and gender with fasting blood sugar control compliance of patients with diabetes mellitus at the Ngraho Health Center. Journal of Health Sciences MAKIA, 12(1):52-59.
20. Kurniawaty, E. (2014). Diabetes mellitus. JUKE, 4(7):114-119.
21. Lumentut, N., Edy, H. J. and Rumondor, E. M. (2020). Formulation and physical stability test of goroho banana peel ethanol extract cream preparation (Musa acuminate L.) with a concentration of 12.5% as sunscreen. Journal of Mathematics and Natural Sciences, 9(2):42-46.
22. Malole, M. B. M. and Pramono, C. S. (1989). Use of Laboratory Experimental Animals. Ministry of Education and Culture, Directorate General of Higher Education, Inter-University Center for Biotechnology. Bogor Agricultural University, Bogor.
23. Medrado, A. R., Pugliese, L. S. Reis, S. R. and Andrade, Z. A. (2003). Influence of low level laser therapy on wound healing and its biological action upon myofibroblasts. Lasers Surg Med, 32:239-244.
24. Modlinska, K. and Pisula, W. (2020). The natural history of model organism : the norway rat, from an obnoxious pest to a laboratory pet. eLife, 9:1-13.
25. Mokoginta, R. V., Simbala, H. E. I. and Mansaudah, K. L. R. (2020). Test of ethanol antioxidant activity of dayak onion bulbus (Eleutherine americana mer) by DPPH method (1,1-diphenyl-2-picrylhydrazyl). Pharmacon, 9(3):451-457.
26. Morisaki, N., Watanabe, S., Tezuka, M., Zenibayashi, M., Shiina, R., Koyama, N., Kanzaki, T. and Saito, Y. (1995). Mechanism of angiogenic effects of saponin from ginseng radix rubra in human umbilical vein endothelial cells. Brit. J. Pharmacol, 115:1188-1193.
27. Nadira, L. A., Jayawardhita, A. A. G. and Adi, A. A. A. M. (2021). The administration of kersen leaf extract ointment is effective in increasing the angiogenesis process in the healing of hyperglycemic mouse skin incision wounds. Indonesia Medicus Veteriner, 10(6):851-860.
28. Nanda, Y., Salim, M. N. and Iskandar, C. D. (2017). Histopathology of the Skin of Mice (Mus musculus) remodelling phase in the healing of cut wounds with jatropha sap ointment (Jatropha curcas Linn). JIMVET, 1(4):780-787.
29. Nawir, A. I., Afifah, C. A. N., Sulandjari, S. and Handajani, S. (2021) Utilization of kersen leaves (Muntingia calabura L.) into the herb. Journal of Culinary Arts, 10(1):1-11.
30. Noor, S. M., Wahuwardani, S., Bakrie, B., Vasito, Praharni, I., Nurhayti, E. S. and Videoti, P. M. (2022). Handling Rodensis in Research According to Animal Welfare Rules. IAARD Press, Jakarta.
31. Nugraha, A. and Muhartono (2013). Comparison of the healing rate of second-degree burns between the administration of topical honey of coffee nectar with silver sulfadiazine in male white rats (Rattus norvegicus) of the Sprague dawley strain. MAJORITY (Medical Journal of Lampung University), 24-32.
32. O’Brien, K., Bhatia, A., Tsen, F., Chen, M., Wong, A. K., Woodley, D. and Li, W. (2014). Identification of the critical therapeutic entity in secreted Hsp90ɑ that promote wound healling in newly re-standardized healthy and diabetic pig models. PloS ONE, 9(12):1- 25.
33. Primadina, N., Basori, A. and Perdanakusuma, D. S. (2019). The wound healing process is reviewed from the aspect of cellular and molecular mechanisms. Qanun Medika, 3(1):31-43.
34. Raju, K. and Balaraman, R. (2008). Antidiabetic mechanism of saponins of Momordica cymbalaria. Phcog Mag, 4(15):197-206.
35. Rizka, A. and Vicky, S. B. (2013). Type 1 collagen density in surgical wounds of wistar rats that were anemic due to acute bleeding. Media Journal Of Emergency. 2(1): 1-12.
36. Rowe, R. C., Paul, J. S. dan Paul, J. W. (2006). Handbook of Pharmaceutical Excipients. Pharmaceutical Press, London.
37. Saryanti, D., Setiawan, I. and Safitri, R. A. (2019). Optimization of the formula of m/a cream preparation from banana peel extract (Musa acuminata L.). Indonesia Journal of Pharmaceutical Research, 1(3): 225- 237.
38. Siregar, S. (2020). The relationship between blood sugar levels during the wound healing process in post-section caesarean  section patients at IPI Medan Hospital. Imelda Scientific Journal of Nursing, 6(1):22-26.
39. Suharto, I. P. S. and Etika, A. N. (2019). Ginger extract (Zingiber Officinale Roscoe) has an effect on the density of collagen fibers in incision wounds. Scientific Journal of Health Sciences, 7(1):27-36.
40. Sumbayak, E. M. (2015). Fibroblasts: their structure and role in wound healing. Journal of Medicine, 21(57).
41. Surjowardojo, P., Sarwiyono, Thohari, I. and Ridhowi, A. (2014). Quantitative and qualitative phytochemical analysis of Muntingia calabura. Journal of Biology, Agriculture and Healthcare, 4(16):84-88.
42. Syahara, S. and Siregar, Y. F. (2019). Phytochemical screening of ethanol extract of kersen leaves (Muntingia calabura). Indonesia Journal of Scientific Health, 4(2):121-125.
43. Taher, M., Zakaria, T. M. F. S. T., Susanti, D. and Zakaria, Z. A. (2016). Hypoglycaemic activity of ethanolic extract of Garcinia mangostana Linn. In normoglycaemic and streptozotocin-induced diabetic rats. BMC Complementary and Alternative Medicine, 16(135):1-12.
44. Tuldjanah, M. Wirawan, W. and Setiawati, N. P. (2020). Test the effect of African leaf ethanol extract (Gymnanthemum amygdalinum (delile) Sch. Bip. Ex Walp) on blood glucose levels of white rats (Rattus norvegicus). Journal of Science and Health, 2(4):340-346.
45. Velnar, T., Bailey, T. and Smrkolj, V. (2009). The wound healing process: an overview of the cellular and molecular mechanism. The Journal of International Medical Research, 37(5):1528-1542.
46. Wilantari, P. D., Santika, A. A. G. J., Buana, K. D. M., Samirana, P.O., Sudimartini, L. M. and Semadi, W. J. (2019). Healing activity of incision wounds from binahong leaf ointment (Anredera scandes (L.) Moq.). Udayana Journal of Pharmacy, 8(2):78-89.
47. Wilson, G. L. (1988). Mechanism of nitroroure induced beta cell damage activation of poly (adp- ribose) syntase and cellular distribution. Diabetes, 37(2):213-216.
48. Wintoko, R. and Yadika, A. D. N. (2020). Up-to-date management of wound care. JK Unila, 4(2):183- 189.
49. Yuda, I. K. A., Anthara, M. S. and Dharmayudha, A. A. G. O. (2013). Identification of a group of chemical compounds of bitter melon ethanol extract (Momordica charantia) and its effect on the decrease in blood glucose levels of aloxan-induced male white rats (Rattus norvegicus). Udayana Veterinary Bulletin, 5(2):87-95.
50. Zhang, M., Lv, X. Y., Li, J., Xu, Z. G. and Chen, L. (2008). The characterization of high-fat diet and multiple low-dose streptozotocin induced type 2 diabetes rat model. Experimental Diabetes Research, 2008:10.