Potential of Lauric Acid, Miristic Acid and Combination Gel as Inhibiting the Growth of Porphyromonas Gingivalis Bacteria in Gingivitis


Authors : Andika Felix Saputra Juang Harefa; Emma Kamelia; Bedjo Santoso; Ari Suwondo, Supriyana

Volume/Issue : Volume 7 - 2022, Issue 12 - December

Google Scholar : https://bit.ly/3IIfn9N

Scribd : https://bit.ly/3QGLZX7

DOI : https://doi.org/10.5281/zenodo.7542590

Abstract : Gingivitis is a mild periodontal disease accompanied by clinical symptoms in the form of red gingiva. The incidence of gingivitis in Indonesia ranks second with 96.58%, while in Central Java it is 25.8%. The main cause of gingivitis is the lack of maintaining oral and dental hygiene, marked by the accumulation of plaque and tartar around the teeth which can be removed by regular brushing and scaling using tools and drugs made from chemicals. The chemicals are considered to have side effects when used for a long time. Many people now choose natural ingredients or herbal ingredients. One of the natural ingredients that can be used for treatment is coconut (Virgin Coconut Oil / VCO). The aim of producing VCO as an alternative ingredient for gingivitis therapy (Laboratory study). This research is a laboratory experiment with a post-test only control group research design. VCO gel is made from fresh coconuts which are processed into VCO by natural fermentation, which is then mixed with NaCMC added Treatanolamin (TEA) and dissolved with glycerin to get VCO in gel form. The research subjects were 24 Rattus Norvegicus. divided into 4 groups, namely the lauric treatment group, the myristic acid treatment group, the combined lauric and myristic acid group and the control group. Experimental animals acclimatized for 5 days were induced by Porphyromonas gingivalis bacteria in LPS (Lipopolysaccharide) preparation in the gingival sulcus of the lower right first incisor as much as 0.01 ml. Experimental animals that have experienced inflammation are then treated with VCO gel. During the fourteen days after drug administration, observations were made on the healing of gingivitis by examining swelling, discoloration of the gingiva, bleeding on the gingiva and histopathological examination using a light microscope with 400x magnification. Post-treatment observation, experimental animals were observed or observed for 14 days. The results of observations are recorded in the observation checklist sheet where the examination of the condition of the gingiva is based on the Modified Gingival Index (MGI). Experimental animals were observed or observed for 14 days. The results of observations are recorded in the observation checklist sheet where the examination of the condition of the gingiva is based on the Modified Gingival Index (MGI). Experimental animals were observed or observed for 14 days. The results of observations are recorded in the observation checklist sheet where the examination of the condition of the gingiva is based on the Modified Gingival Index (MGI).Results:virgin coconut oil (lauric acid) is effective against killing and weakening Porphyromonas gingivalis bacteria. Virgin coconut oil (myristic acid) is effective against killing and weakening Porphyromonas gingivalis bacteria, but lauric acid is more effective. Virgin coconut oil (a combination of lauric acid and myristic acid) is effective against killing and weakening Porphyromonas gingivalis bacteria, but more effective than lauric acid and myristic acid. Conclusion: virgin coconut oil is effective as an alternative ingredient to inhibit Porphyromonas gingivalis bacteria in gingivitis. The lauric acid group is more effective than the myristic acid group, combinednor control.

Keywords : Gingivitis, Virgin Coconut Oil, Porphyromonas gingivalis bacteria

Gingivitis is a mild periodontal disease accompanied by clinical symptoms in the form of red gingiva. The incidence of gingivitis in Indonesia ranks second with 96.58%, while in Central Java it is 25.8%. The main cause of gingivitis is the lack of maintaining oral and dental hygiene, marked by the accumulation of plaque and tartar around the teeth which can be removed by regular brushing and scaling using tools and drugs made from chemicals. The chemicals are considered to have side effects when used for a long time. Many people now choose natural ingredients or herbal ingredients. One of the natural ingredients that can be used for treatment is coconut (Virgin Coconut Oil / VCO). The aim of producing VCO as an alternative ingredient for gingivitis therapy (Laboratory study). This research is a laboratory experiment with a post-test only control group research design. VCO gel is made from fresh coconuts which are processed into VCO by natural fermentation, which is then mixed with NaCMC added Treatanolamin (TEA) and dissolved with glycerin to get VCO in gel form. The research subjects were 24 Rattus Norvegicus. divided into 4 groups, namely the lauric treatment group, the myristic acid treatment group, the combined lauric and myristic acid group and the control group. Experimental animals acclimatized for 5 days were induced by Porphyromonas gingivalis bacteria in LPS (Lipopolysaccharide) preparation in the gingival sulcus of the lower right first incisor as much as 0.01 ml. Experimental animals that have experienced inflammation are then treated with VCO gel. During the fourteen days after drug administration, observations were made on the healing of gingivitis by examining swelling, discoloration of the gingiva, bleeding on the gingiva and histopathological examination using a light microscope with 400x magnification. Post-treatment observation, experimental animals were observed or observed for 14 days. The results of observations are recorded in the observation checklist sheet where the examination of the condition of the gingiva is based on the Modified Gingival Index (MGI). Experimental animals were observed or observed for 14 days. The results of observations are recorded in the observation checklist sheet where the examination of the condition of the gingiva is based on the Modified Gingival Index (MGI). Experimental animals were observed or observed for 14 days. The results of observations are recorded in the observation checklist sheet where the examination of the condition of the gingiva is based on the Modified Gingival Index (MGI).Results:virgin coconut oil (lauric acid) is effective against killing and weakening Porphyromonas gingivalis bacteria. Virgin coconut oil (myristic acid) is effective against killing and weakening Porphyromonas gingivalis bacteria, but lauric acid is more effective. Virgin coconut oil (a combination of lauric acid and myristic acid) is effective against killing and weakening Porphyromonas gingivalis bacteria, but more effective than lauric acid and myristic acid. Conclusion: virgin coconut oil is effective as an alternative ingredient to inhibit Porphyromonas gingivalis bacteria in gingivitis. The lauric acid group is more effective than the myristic acid group, combinednor control.

Keywords : Gingivitis, Virgin Coconut Oil, Porphyromonas gingivalis bacteria

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