Authors : Alsira Salsa Hayunda; Nin Suharti Hasibuan; Liza Mutia; Sukaisi; Febri Sembiring

Volume/Issue : Volume 10 - 2025, Issue 5 - May

Google Scholar : https://tinyurl.com/4uwewwkz

DOI : https://doi.org/10.38124/ijisrt/25may498

Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.

Abstract : Increased environmental awareness among commercial dyes and textile exporters is shifting attention to the use of natural dyes pigments in foodstuffs, pharmaceuticals and textile products. This is because synthetic dyes are relatively expensive and cause reactions such as allergies or are carcinogenic which are potentially harmful to the environment and human health. One of the natural dyes that is usually used in staining is henna leaves (Lawsonia inermis). This study aims to determine the potential of henna leaves extract in staining Escherichia coli and Salmonella typhi. The type of research used is descriptive qualitative to observe the effectiveness of henna leaves extract in staining bacteria with an experimental method that aims to compare the results of staining between safranin control and henna leaves extract. Based on the research conducted, it was found that only henna leaves extract with a concentration of 100% was able to staining Escherichia coli and Salmonella typhi bacteria like safranin with brown color, while the results of henna leaves extract oxidized using potassium permanganate and sodium carbonate could not dyeing the two bacteria properly and clearly. So further research is needed on Lawsonia inermis leaves extract to be able to dyeing other bacteria.

Keywords : Alternative Dyes; Henna Leaf Extract; Escherichia coli; Salmonella Typhi.

References :

1. Adisa, J. O., Musa, K. K., Egbujo, E. C. & Uwaeme, I. M., 2017. A Study of Various Modifications of Lawsonia inermis (Henna) Leaf Extract as A Cytoplasmic Stain in Liver Biopsies. International Journal of Research in Medical Sciences, 5(3), pp. 1058-1065.
2. Asfiya, N. A., Novalina, D. & Astuti, T. D., 2024. Potensi Dan Uji Stabilitas Ekstrak Lawsonia Inermis Sebagai Cat Penutup Pada Gram Staining Dengan Variasi Suhu. Borneo Journal Of Medical Laboratory Technology, 6(2), p. 540–546.
3. Chukwu, O. et al., 2011. Application of Extracts of Henna (Lawsonia inamis) Leaves as A Counter Stain. African Journal of Microbiology Research, V(21), pp. 3351-3356.
4. Dafrita, I. E. & Sari, M., 2020. Senduduk dan Ubi Jalar Ungu Sebagai Pewarna Preparat Squash Akar Bawang Merah. JPBIO (Jurnal Pendidikan Biologi), V(1), p. 46–55.
5. Hafiz, H., O.O.C, C. & and Nura, S., 2012. The Potensials of Henna (Lawsonia Inamis L.) Leaves Extracts as Counter Stain in Gram Staining Reaction. Bayero Journal of Pure and Applied Sciences, V(2), p. 56–60 .
6. Indrawati, R., Jenny, G. & Tumpuk, S., 2022. Senggani Fruit Anthocyanins (Melastoma Malabathricum Auct, Non-Linn) as Bacterial Dyes Differential Painting Techniques. INTEK Jurnal Penelitian, 9(1), pp. 18-24.
7. Jannah, Y. N., Islawati & Asriyani, 2023. Pemanfaatan Ekstrak Daun Bayam Merah (Althernantera Amoena Voss) sebagai Pewarna Alami pada Pewarnaan Bakteri Klebsiella Pneumoniae. Nuhela Journal of Injury, II(2), pp. 154-162.
8. Khantamat, O. et al., 2021. Safety and Bioactivity Assessment of Aqueous Extract of Thai Henna (Lawsonia inermis Linn.) Leaf. Journal of Toxicology and Environmental Health, Part A, 84(7), pp. 298-312.
9. Niken & Yulia, I., 2023. Innovation of Extract (Lawsonia Inermis L) as Alternative Dye for Escherichia Coli Bacterial Staining. International Journal of Multidisciplinary Approach Research and Science, I(3), pp. 512-517.
10. Papalanggi, K. M. & Dwisari, F., 2023. Pengaruh Perbedaan Variasi Waktu Rendaman Daun Bayam Merah (Amaranthus Tricolor L.) Sebagai Alternatif Pewarna Pengganti Safranin Pada Pemeriksaan Bakteri Escherichia Coli. Pontianak, Politeknik Aisyiyah Pontianak.
11. Prastiwi, N. P., 2021. Ekstrak Etanol Daun Pacar (Lawsonia Inermis L.) Sebagai Pewarna Alternatif Safranin Untuk Pewarnaan Klebsiella Pneumoniae. Surabaya, University of Nahdlatul Ulama Surabaya.
12. Saputra, B. E., Bintari, Y. R. & Risandiansyah, R., 2022. Uji Validasi Akurasi dan Presisi Metode Pewarnaan Sederhana Staphylococcus aureus dan Escherichia coli Menggunakan Ekstrak Metanolik Hibiscus sabdariffa Linn. Jurnal Bio Komplementer Medicine, 9(1).
13. Singh, S. et al., 2015. Safety and Efficacy of Hydroalcoholic Extract from Lawsonia inermis Leaves on Lipid Profile in Alloxan Induced Diabetic Rats. AYU, 36(1), pp. 107-112.
14. Venturina, S. D. T., Comuelo, J. Z. A., Samaniego, W. R. A. & Jolito, F. C., 2020. The Utilization of Methanolic Bixa orellana (Annatto) Seed Extract as Substitute for Safranin in Gram Staining. Publiscience, III(1), pp. 33-36.
15. Virgianti, D. P. & Luciana, C., 2017. Penggunaan Ekstrak Kombinasi Angkak dan Daun Jati Sebagai Pewarna Penutup pada Pewarnaan Gram. Jurnal Kesehatan Bakti Tunas Husada , 17(1), pp. 66-72.
16. Yusuf, M. & Mohammad, F., 2017. Natural Colorant from Lawsonia Inermis Leaves: Reflectance Spectroscopy-Induced Optimal Conditions of Extraction and Dyeing. Scrivener Publishing LLC, p. 89–102.