Authors : Jared Onyancha; Denis Menge; Elias Mandela; Peter Maloba; Sydney Wanjiru; Sammy Kimoloi

Volume/Issue : Volume 9 - 2024, Issue 6 - June

Google Scholar : https://tinyurl.com/5dvue7xb

Scribd : https://tinyurl.com/5d85naz9

DOI : https://doi.org/10.38124/ijisrt/IJISRT24JUN1413

Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.

Abstract : Fagaropsis angolensis is widely used in African traditional medicine system to manage oxidative stress-associated diseases and lacks scientific evidence. The study investigated the phytochemical, antioxidant, anti-nociceptive and anti-inflammatory properties of F. angolensis leaf extracts. Extracts were prepared by maceration and standard qualitative methods were used for phytochemical screening. Total phenolic and total flavonoid contents were done by using Folin-Ciocalteu and aluminum chloride calorimetricmethods, respectively. Antioxidant activity was evaluated using 2, 2-Diphenyl-1- picrylhydrazyl (DPPH) method. Acetic acid-induced writhing and carrageenan-induced hind paw edema mouse models were used in anti-nociceptive and anti- inflammatory activities respectively. The presence of steroids, phenols, alkaloids, flavonoids, anthraquinones, glycoside and coumarins were observed. Total phenolic content for methanol extract was 55.52 ± 3.05 and the water extract was 48 ± 0.185 mgGAE/g (p<0.05). While, total flavonoid content for methanol extract was 172.53 ± 7.095 and that of water extracts was 42.23 ± 0.101 mgCE/g (p<0.05). IC50 values of less than 1 μg/ml were revealed in the DPPH assay. Percentage (%) writhing inhibition did not show any difference between the tested doses of plant extracts and standard aspirin at 150 mg/kg (p>0.05), indicative of potent anti-nociceptive activity. There was no significant difference in percentage paw edema inhibition between the plant extracts and the dexamethasone standard at 10 mg/kg (p>0.05), indicative of potent anti-inflammatory activity.This study adds to existing knowledge about the utilization of F. angolensis in traditional medicine for the management of related to oxidative stress including pain and inflammatory reactions.

Keywords : DPPH Assay; Medicinal Plants; Phenolic Content; Rheumatoid.

References :

1. Q. Liu, Y. Gao, W. Dong, and L. Zhao, “Plastome evolution and phylogeny of the tribe Ruteae (Rutaceae),” Ecol. Evol., vol. 13, no. 2, pp. 1–20, 2023, doi: 10.1002/ece3.9821.
2. E. S. Mutinda et al., “Traditional medicinal uses, pharmacology, phytochemistry, and distribution of the Genus Fagaropsis (Rutaceae),” J. Ethnopharmacol., vol. 284, no. October 2021, p. 114781, 2022, doi:10.1016/j.jep.2021.114781.
3. J. Pascaline, M. Charles, O. George, C. Lukhoba, L. Ruth, and M. S. D, “Ethnobotanical survey and propagation of some endangered medicinal plants from south Nandi district of Kenya.,” J. Anim. Plant Sci., vol. 8, no. 3, pp. 1016–1043, 2010
4. O. Jared Misonge, G. Nicholas Kamindu, W. Sabina Wangui, G. Michael Muita, and C. Onyancha Jared Misonge, “An ethnobotanical survey of plants used for the treatment and management of cancer in Embu County, Kenya,” J. Med. Plants Stud., vol. 7, no. 4, pp. 39–46, 2019.
5. J. Mukavi et al., “Anti-inflammatory norhopanes from the root bark of Fagaropsis angolensis (Engl.) H.M.Gardner,” Fitoterapia, vol. 146, no. July, p. 104690, 2020, doi: 10.1016/j.fitote.2020.104690.
6. H. J. Beentje, Kenya Trees, Shrubs and Lianas. Nairobi: National Museums of Kenya, 1994.
7. M. Maundu, Traditional food plants of Kenya. Kenya. Nairobi, Kenya: National Museums of Kenya, 1999.
8. S. N. Wambugu, P. M. Mathiu, D. W. Gakuya, T. I. Kanui, J. D. Kabasa, and S. G. Kiama, “Medicinal plants used in the management of chronic joint pains in Machakos and Makueni counties, Kenya,” J. Ethnopharmacol., vol. 137, no. 2, pp. 945–955, 2011, doi: 10.1016/j.jep.2011.06.038.
9. J. Kokwaro, Medicinal Plants of East Africa: Third Edition. University of Nairobi Press: Nairobi, Kenya. 2009.
10. J. Pascaline, M. Charles, O. George, and C. Lukhoba, “An inventory of medicinal plants that the people of Nandi use to treat malaria,” J. Anim. Plant Sci., vol. 9, no. 3, pp. 1192–1200, 2011.
11. D. Louppe, A. Oteng-Amoako, and M. Brink, Eds., Plant Resources of Tropical Africa 7 (1), Timber 1. PROTA Foundation Wageningen, Nertherlands/ Backhuys Publishers, Leiden, Netherlands/CTA, Wageningen, Netherlands. pp. 784-785, 7(1). 2008.
12. Itonga Stanely Mwobia, “Ethnobotanical Study of Plants Used in Traditional and biopesticides in Meru Central Kenya and preliminary toxicological evaluation of Tephrosia vogelii. Msc. Thesis of the University of Nairobi,” 2011.
13. Ken F., “Useful Tropical Plants Database Web InterfaCE BY Ajna Fern and RichardMorris. Tropical ferns. Info,” 2014.
14. N. Shiracko, B. O. Owuor, M. M. Gakuubi, and W. Wanzala, “A survey of ethnobotany of the AbaWanga people in Kakamega County, western province of Kenya,” Indian J. Tradit. Knowl., vol. 15, no. 1, pp. 93–102, 2016.
15. C. N. Muthaura, G. M. Rukunga, S. C. Chhabra, G. M. Mungai, and E. N. M. Njagi, “Traditional phytotherapy of some remedies used in treatment of malaria in Meru district of Kenya,” South African J. Bot., vol. 73, no. 3, pp. 402–411, 2007, doi: 10.1016/j.sajb.2007.03.004.
16. P. G. Kareru, G. M. Kenji, A. N. Gachanja, J.M. Keriko, and G. Mungai, “Traditional medicines among the Embu and Mbeere peoples of Kenya,” African J. Tradit. Complement. Altern. Med., vol. 4, no. 1, pp. 75–86, 2007.
17. G. Kewessa, T. Abebe, and A. Demessie, “Indigenous knowledge on the use and management of medicinal trees and shrubs in Dale District, Sidama Zone, Southern Ethiopia,” Ethnobot. Res. Appl., vol. 14, no. June, pp. 171–182, 2015, doi: 10.17348/era.14.0.171-182.
18. Y. S. Birhan, Medicinal plants utilized in the management of epilepsy in Ethiopia: ethnobotany, pharmacology and phytochemistry, vol. 17, no. 1. BioMed Central, 2022. doi: 10.1186/s13020-022-00686-5.
19. S. Asnake, T. Teklehaymanot, A. Hymete, B. Erko, and M. Giday, “Survey of Medicinal Plants Used to Treat Malaria by Sidama People of Boricha District, Sidama Zone, South Region of Ethiopia,” Evidence-based Complement. Altern. Med., vol. 2016, 2016, doi: 10.1155/2016/9690164.
20. Almaz Sematu, “Extraction of essential oils from Fagaropsis angolensis, Callistemon citrinus and Melaleuca armillaris and assessment of their antimicrobial and insecticidal activities. Msc. Thesis of Jimma University, Ethiopia,” 2021.
21. D. Lacroix et al., “Antiplasmodial and cytotoxic activities of medicinal plants traditionally used in the village of Kiohima, Uganda,” J. Ethnopharmacol., vol. 133, no. 2, pp. 850–855, 2011, doi: 10.1016/ j.jep.2010.11.013.
22. M. Kuglerova et al., “Antimicrobial and antioxidative effects of Ugandan medicinal barks,” African J. Biotechnol., vol. 10, no. 18, pp. 3628–3632, 2011.
23. P. G. Kirira et al., “Anti-plasmodial activity and toxicity of extracts of plants used in traditional malaria therapy in Meru and Kilifi Districts of Kenya,” J. Ethnopharmacol., vol. 106, no. 3, pp. 403–407, 2006, doi: 10.1016/j.jep.2006.01.017.
24. G. Gaobotse et al., “The use of African medicinal plants in cancer management,” Front. Pharmacol., vol. 14, no. February, pp. 1–22, 2023, doi: 10.3389/fphar.2023.1122388.
25. Β. K. Alemu and D. Misganaw, "Antimalarial Activity of Fagaropsis angolensis (Rutaceae) Crude Extracts and Solvent Fractions of Its Stem Bark Against Plasmodium berghei in Mice," J. Exp. Pharmacol., vol. 12, pp. 683-693, 2020, doi: 10.2147/ JEP.S289478.
26. J. M. Onyancha, N. K. Gikonyo, S. W. Wachira, P. G. Mwitari, and M. M. Gicheru, "Anticancer activities and safety evaluation of selected Kenyan plant extracts against breast cancer cell lines," J. Pharmacogn. Phyther., vol. 10, no. 2, pp. 21-26, 2018, doi: 10.5897/JPP2017.0465.
27. M. J. Onyancha, "Evaluation of antibreast and antiprostate cancer activities of selected Medicinal plants from some parts of Kenya. PhD Thesis of Kenyatta University," 2019. [Online]. Available: https://ir- [ library.ku.ac.ke/bitstream/handle/ 123456789/ 21221/Evaluation of antibreast and antiprostate.....pdf?sequence=1
28. E. Tajbakhsh, T. E. Kwenti, P. Kheyri, S. Nezaratizade, D. S. Lindsay, and F. Khamesipour, "Antiplasmodial, antimalarial activities and toxicity of African medicinal plants: a systematic review of literature," Malar. J., vol. 20, no. 1, 2021, doi: 10.1186/s12936-021-03866-0.
29. Mukavi Justus Wambua, "Anticancer and antiinflammatory secondary metabolites of Fagaropsis angolensis (ENGL. Msc. Thesis of the University of Nairobi," 2020.
30. A. L. Yiaile, J. M. Mbaria, I. M. Ole- [Mapenay, M. O. Okumu, A. H. Hadun, and J. M. Onyancha, "Preliminary Screening of Crude Extracts of Fagaropsis Angolensis for Anticancer Activity," Pharmacogn. Commun., vol. 8, no. 2, pp. 75-80, 2018, doi: 10.5530/pc.2018.2.15.
31. M. C. M, M. J. C, and V. J. M, "Larvicidal compounds from Fagaropsis angolensis leaves against malaria vector (Anopheles gambiae)," Int. J. Malar. Res. Rev. www.resjournals.org/IJMR, vol. 1, no. 1, pp. 1-7, 2013, [Online]. Available: www.resjournals.org/ IJMR
32. Mudalungu Cynthia Muhavi, "Mosquito larvicidal compounds from the plant Fagaropsis angolensis (Engl. Dale) against Anopheles gambiae. Msc. Thesis of Egerton University," 2013. [Online]. Available: http://dx.doi.org/10.1016/j.jsames.2011.03.00 3%0A https://doi.org/10.1016/j.gr.2017.08.001%0Ahttp://dx.doi.org/10.1016/j.precamres.2014.12.018%0Ahttp://dx.doi.org/10.1016/j.precamres.2011.08.005% 0A http://dx.doi.org/10.1080/00206814.2014.902757% 0Ahttp://dx.
33. P. G. Waterman and S. A. Khalid, "The biochemical systematics of Fagaropsis angolensis and its significance in the Rutales," Biochem. Syst. Ecol., vol. 9, no. 1, pp. 45-51, 1981, doi: 10.1016/0305- 1978(81)90058-2.
34. W. C. Evans, Trease and Evans' Pharmacognosy: Sixteenth Edition. Elsevier Inc., 2009.
35. J. B. Harborne, Textbook of Phytochemical Methods. A Guide to Modern Techniques of Plant Analysis. 5th Edition, Chapman and Hall Ltd, London. 1998.
36. G. O. Sengera, E. O. Kenanda, and J. M. Onyancha, "Antibacterial, Antioxidant Potency, and Chemical Composition of Essential Oils from Dried Powdered Leaves and Flowers of Hypericum revolutum subsp. keniense (Schweinf.)," Evidence-based Complement. Altern. Med., vol. 2023, 2023, doi: 10.1155/2023/4125885.
37. P. Maloba, E. Mandela, S. Kimoloi, and J. Onyancha, "Antiinflammatory Antinociceptive and Antioxidant activities of aqueous and methanolic stem bark extracts of Fagaropsis hildebrandtii (Engl.) Milne-Redh . (Rutaceae)," J Pharmacol, vol. 12, no. 4, pp. 211-217, 2023, 10.31254/phyto.2023.12401. doi:
38. N. Saeed, M. R. Khan, and M. Shabbir, "Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L," BMC Complement. Altern. Med., vol. 12, 2012, doi: 10.1186/1472-6882-12-221.
39. J. Cheng et al., "In in vivo evaluation of the anti-inflammatory and analgesic activities of compound Muniziqi granule in experimental animal models," BMC Complement. Altern. Med., vol. 16, no. 1, pp. 1-10, 2016, doi: 10.1186/s12906-016-0999-y.
40. B. M. Wangusi, L. W. Kanja, I. M. Ole- Mapenay, and J. M. Onyancha, "Acute Toxicity, Phytochemical Screening, Analgesic, and Anti-Inflammatory Activities of Aqueous and Methanol Root Extracts of Maerua triphylla A. Rich. (Capparaceae)," Evidence-based Complement. Altern. Med., vol. 2021, 2021, doi: 10.1155/2021/3121785.
41. M. A. Rahman, J. S. Chakma, S. Islam, M. S. Rana, and N. U. Ahmed, "Analgesic and anti-inflammatory effect of Clausena suffruticosa root extract in animal model," J. Sci. Res., vol. 3, no. 3, pp. 631-639, 2011, doi: 10.3329/jsr.v3i3.7594.
42. T. Yimer, E. M. Birru, M. Adugna, M. Geta, and Y. K. Emiru, "Evaluation of analgesic and anti-inflammatory activities of 80% methanol root extract of echinops kebericho m. (asteraceae)," J. Inflamm. Res., vol. 13, pp. 647-658, 10.2147/JIR.S267154. 2020, doi:
43. S. Bindu, S. Mazumder, and U. Bandyopadhyay, "Non-steroidal anti- inflammatory drugs (NSAIDs) and organ damage: A current perspective." Biochem. Pharmacol., vol. 180, no. January, 2020, doi: 10.1016/j.bcp.2020.114147.
44. O. Josephine Ozioma, E.-O., & Antoinette Nwamaka Chinwe, "Herbal Medicines in African Traditional Medicine," in Herbal Medicine, 2019. [Online]. Available: http://dx.doi.org/10.1039/C7RA00172 J%0Ahttps://www.intechopen.com/books/advanced-biometric-technologies/liveness-detection-in-biometrics%0Ahttp://dx.doi.org/10.1016/j.colsurfa. 2011.12. 014
45. F. Orozco Montes, A. Vázquez-Hernández, and B. Fenton-Navarro, "Active compounds of medicinal plants, mechanism for antioxidant and beneficial effects Orozco," Rev. Int. Botánica Exp. Int. J. Exp. Bot., vol. 9457, pp. 1-154, 2019.
46. R. M. H. Shoker, "A Review Article: The Importance of the Major groups of Plants Secondary Metabolism Phenols, Alkaloids, and Terpenes," Int. J. Res. Appl. Sci. Biotechnol., vol. 7, no. 5, pp. 354-358, 2020, doi: 10.31033/ijrasb.7.5.47.
47. M. Marčetić and J. Arsenijević, "Antioxidant [ activity of plant secondary metabolites," Arh. Farm. (Belgr)., vol. 73, no. 4, pp. 264-277, 2023, doi: 10.5937/arhfarm73-45560.
48. M. H. Ibrahim, H. Z. E. Jaafar, E. Karimi, and A. Ghasemzadeh, "Allocation of secondary metabolites, photosynthetic capacity, and antioxidant activity of Kacip Fatimah (Labisia pumila benth) in response to CO2 and light intensity," Sci. World J., vol. 2014, 2014, doi: 10.1155/2014/360290.
49. M. A. Ayanaw, J. S. Yesuf, and E. M. Birru, "Evaluation of Analgesic and Anti- inflammatory Activities of Methanolic Leaf and Root Extracts of Gomphocarpus purpurascens A. Rich (Asclepiadaceae) in Mice," J. Exp. Pharmacol., vol. 15, no. January, pp. 1-11, 2023, doi: 10.2147/JEP.S361194.
50. B. Olela, J. Mbaria, T. Wachira, and G. Moriasi, "Acute Oral Toxicity and Anti- inflammatory and Analgesic Effects of Aqueous and Methanolic Stem Bark Extracts of Piliostigma thonningii (Schumach.)," Evidence-based Complement. Altern. Med., vol. 2020, 2020, doi: 10.1155/2020/5651390.
51. Μ. Τ. Mansouri, A. A. Hemmati, B. Naghizadeh, S. A. Mard, A. Rezaie, and B. Ghorbanzadeh, "A study of the mechanisms underlying the anti-inflammatory effect of ellagic acid in carrageenan-induced paw edema in rats,” Indian J. Pharmacol., vol. 47, no. 3, pp. 292–298, 2015, doi: 10.4103/0253-7613.157127