Performance of Serum Tube Agglutination Test and Enzyme-Linked Immunosorbent Assay IgG and IgM Tests for Diagnosis of Human Brucellosis among Employees of Widam Slaughter Establishments


Authors : Maisan Ahmed, Samirha Hamid Ablelrahman, Adam Dawoud Abakar, Yousif Abdelhameed Mohammed, Dr. Elmoubasher Abu Baker Abd Farag, Wassan Abdullah Al-baker, Hamad Eid Al-Romaihi, Mohamed Hamad Al-Thani

Volume/Issue : Volume 4 - 2019, Issue 8 - August

Google Scholar : https://goo.gl/DF9R4u

Scribd : https://bit.ly/2kotJqy

 Objectives Brucellosis is reported as important food borne zoonotic infection worldwide. There are number of techniques utilize to detect the presence of Brucella species in human sera and these techniques have different levels of specificity and sensitivity and the purpose of this study is to assess the Diagnostic Performance of Serum Tube Agglutination Test and Enzyme-Linked Immunosorbent Assay IgG and IgM Tests for Diagnosis of Human Brucellosis among the study group at Widam slaughter establishments.  Methods A total of 141 human sera samples were collected from large animals’ slaughter establishments in Qatar, namely, Abu Hamour slaughter establishment, Alshamal Slaughter establishment, Muaither slaughter establishment, Abu Hamour slaughterhouse for the Public, Um Salal slaughterhouse, AlMazroua slaughterhouse, Al Rayan slaughterhouse, Al shahaniya slaughterhouse from August 2017 to September 2017. The samples were tested by serological tests, serum tube agglutination test (STAT), and enzyme-linked immunosorbent assay (ELISA) (immunoglobulin M [IgM] and immunoglobulin G [IgG]).  Results 130 participants were divided into two age groups (18 - 45 and ≥ 46), in which, 82.3% were aged 18 – 45 years and 17.7% were aged ≥ 46 yrs. The age range across the samples was 18-68 years with a mean age of 38.35 Years (SD = 8.62). Additionally, the majority of the participants were male (99.2%, n=129) and 77.7% (n= 101) were married. Overall, 73.1% (n=95) were from South East Asian Countries, followed by 26.1% (n=34) from Arab African Countries. ELISA IgG and IgM has sensitivity (83.4% and 16.67%) respectively and specificity (93.3% and 99.13%).  Conclusion Conclusion that ELISA IgM tests should not be used to confirm brucellosis.

Keywords : Brucellosis, STAT, ELISA, IgG and IgM, Qatar.

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