Optimizing Bioethanol Production through Separated Hydrolysis Fermentation of Rubber-Cassava (Manihot Glaziovii) Starch with K+ and Mg2+ Mineral Ion Supplements


Authors : Hargono; Andreas Kevin Santoso; Aurellia Livia Hidayat; Auxensius Rexer Fransenda; Abram Hasiholan Marpaung; Steffanie Glory Sihombing

Volume/Issue : Volume 8 - 2023, Issue 10 - October

Google Scholar : https://tinyurl.com/2apy4b9n

Scribd : https://tinyurl.com/28nhz3a2

DOI : https://doi.org/10.5281/zenodo.10090855

Abstract : This study focuses on the production of bioethanol from rubber cassava starch using hydrolysis and fermentation processes. The research includes the impact of substrate concentration on the concentration of reducing sugars in hydrolysis, the influence of mineral ion concentrations of K+ and Mg2+ in the final fermentation on bioethanol yield, the effect of substrate concentration on bioethanol yield, and the influence of the final fermentation time on bioethanol yield. The results indicate that increasing substrate concentration leads to higher concentrations of reducing sugars due to more substrate molecules reacting with enzymes. Higher concentrations of mineral ions K+ and Mg2+ during fermentation also result in increased bioethanol yield, as these ions enhance yeast cell growth and metabolism, accelerating the fermentation process. Additionally, higher substrate concentrations positively affect bioethanol yield because more reducing sugars are available for yeast cells to convert into ethanol. Longer final fermentation times also increase bioethanol yield, as they facilitate microorganism performance in converting reducing sugars into bioethanol. However, excessively prolonged fermentation times should be avoided to prevent microbial cell death due to nutrient depletion and carbon dioxide toxicity.

Keywords : Bioethanol, Rubber Cassava, Mineral Ions, Alternative Energy, Starch.

This study focuses on the production of bioethanol from rubber cassava starch using hydrolysis and fermentation processes. The research includes the impact of substrate concentration on the concentration of reducing sugars in hydrolysis, the influence of mineral ion concentrations of K+ and Mg2+ in the final fermentation on bioethanol yield, the effect of substrate concentration on bioethanol yield, and the influence of the final fermentation time on bioethanol yield. The results indicate that increasing substrate concentration leads to higher concentrations of reducing sugars due to more substrate molecules reacting with enzymes. Higher concentrations of mineral ions K+ and Mg2+ during fermentation also result in increased bioethanol yield, as these ions enhance yeast cell growth and metabolism, accelerating the fermentation process. Additionally, higher substrate concentrations positively affect bioethanol yield because more reducing sugars are available for yeast cells to convert into ethanol. Longer final fermentation times also increase bioethanol yield, as they facilitate microorganism performance in converting reducing sugars into bioethanol. However, excessively prolonged fermentation times should be avoided to prevent microbial cell death due to nutrient depletion and carbon dioxide toxicity.

Keywords : Bioethanol, Rubber Cassava, Mineral Ions, Alternative Energy, Starch.

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