Authors :
Ingilala Satheesh Kumar ; Nadakuditi Venkata Raju; Hanumohan R. Konatham
Volume/Issue :
Volume 5 - 2020, Issue 6 - June
Google Scholar :
http://bitly.ws/9nMw
Scribd :
https://bit.ly/3fo6qo7
DOI :
10.38124/IJISRT20JUN851
Abstract :
RAPD-PCR was employed for the
characterization of S. aureus isolates from different
sources such as soil, water, milk, meat and skin swab
etc.,. Isolated pure cultures of S.aureus strains were
subjected to genomic DNA isolation, purification,
separation and quantification. Isolated DNA samples
were distinguished by using 4 different random
primers. Genome profile analysis obtained from the
S.aureus demonstrated that it was possible to
differentiate the S. aureus strains from different sources
by RAPD technique. Results indicate possible
relationships between host origin and genetic variation
among S. aureus isolates from various sources. This
RAPD method was thus useful for epidemiological
studies of the S. aureus flora.
Keywords :
Staphylococcus aureus, Genomic DNA, Random Amplified Polymorphic DNA (RAPD) and Polymerase Chain Reaction (PCR)
RAPD-PCR was employed for the
characterization of S. aureus isolates from different
sources such as soil, water, milk, meat and skin swab
etc.,. Isolated pure cultures of S.aureus strains were
subjected to genomic DNA isolation, purification,
separation and quantification. Isolated DNA samples
were distinguished by using 4 different random
primers. Genome profile analysis obtained from the
S.aureus demonstrated that it was possible to
differentiate the S. aureus strains from different sources
by RAPD technique. Results indicate possible
relationships between host origin and genetic variation
among S. aureus isolates from various sources. This
RAPD method was thus useful for epidemiological
studies of the S. aureus flora.
Keywords :
Staphylococcus aureus, Genomic DNA, Random Amplified Polymorphic DNA (RAPD) and Polymerase Chain Reaction (PCR)
