Lacto ferrin is an iron chelating glycoprotein of the transferrin family present in several biological fluids. The study did a molecular identification, quantitative analysis of LF from Camel, Goat and Sheep milk andalso investigated the effect of the purified LF on the growth inhibition of Staphylococcus aureus and Escherichiacoli. Lactoferrin, was isolated from camel, goat and sheep milk. Both isolation and subsequent purification of the Lactoferrin from these sources using CM Sephadex C-50, showed that camel milk contained appreciable quantities of LF (2.60mg/ml).While Goat milk and Sheep milk contained less quantities of LF as compared to Camel milk (2.10mg/ml and 1.70mg/ml) respectively. The result of bactericidal activity demonstrate that Camel LF is bacteriocidal towards S.aureus and E.coli. Minimal Inhibitory Concentrations were seen even at higher concentrations and the least concentration was at 0.60mg/ml of camel LF against E.coli and 0.2mg/ml of camel LF against S.aureus. The findings of this study Suggest that lactoferrin has inhibitory potential against S. aureus and E. coli.