Authors : Faiza Abdalla Sharif HajEdris; Sulieman Mohammed Elsanousi; Duaa Hajali Hajhamad Ibrahim; Magdi Babiker Omer Mohamed; Hussein Ali Ahmed Ghanim

Volume/Issue : Volume 11 - 2026, Issue 7 - July

Google Scholar : https://tinyurl.com/mtws77yw

Scribd : https://tinyurl.com/mr3n5hvx

DOI : https://doi.org/10.38124/ijisrt/26jul275

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Abstract : Shiga toxin-producing Escherichia coli (STEC) and extended-spectrum β-lactamase (ESBL)-producing strains are a major threat to paediatric health, especially in resource-limited settings. The present study was conducted to investigate the molecular characteristics of Shiga toxin genes (stx1 and stx2) and ESBL genes (blaCTX, blaTEM, blaSHV) in Escherichia species isolated from children with diarrhea, Kassala State, Sudan. 2. A descriptive cross sectional study was conducted from the year 2021-2024 among 404 children less than five years of age presenting with diarrhoea. Stool specimens were cultured on MacConkey agar and E. coli isolates were identified by conventional microbiological MicroScan system was used for determination of antimicrobial susceptibility. Multiplex PCR was used for the detection of Shiga toxin genes (stx1, stx2) and extended spectrum beta-lactamase (ESBL) genes (blaCTX, blaTEM, blaSHV).

Keywords : Escherichia Coli; Shiga Toxin; stx1; stx2; ESBL; Antimicrobial Resistance; Children; Sudan.

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