A study to distinguish the seed-borne fungi
of non-disinfected and disinfected soybean seeds was
conducted in Etay El-Baroud Agricultural Research
Station, El-Beheira Governorate, Egypt during 2017. A
total of seven fungi namely; Aspergillus flavus, A.
parasiticus (Aspergillus flavus group), A. niger, Rhizopus
stolonifer, Pythium spp., Fusarium spp., Alternaria spp.
and Penicillium spp. were secluded which varied in
there frequency. Also, qualitative levels of aflatoxin(s)
produced by fifty isolates of A. flavus group from ten
locations at El-Beheira Governorate were described by
using fluorescence technique. Blue and green
fluorescence surrounding the colonies of A. flavus
group was observed in 32 out of the 50 isolates. Three
isolates of A. flavus group; 1) highly producer, 2)
moderately producer and 3) non producer as examples,
were studied genetically using Polymerase Chain
Reaction (PCR) based assess with versicolorin A
dehydrogenase (ver-1), norsolorinic acid reductase
(nor-1) sterigmatocystin O-methyltransferase (omt-A)
and aflatoxin R (aflR) primers. Generally, for the tested
isolates there is a complete form with four bands
obtained in PCR. The occurrence of patterns in the
non-aflatoxinogenic isolates shows that PCR is not a
satisfactory marker for distinction between
aflatoxinogenic and some non-aflatoxinogenic isolates,
but PCR exhibited that it is speedy and sensitive
technique for revealing fungi.
Keywords : Seed Borne Fungi, PCR, Aflatoxins, Aspergillus Flavus Group, Soybean.