Authors : Kumar Manikanta; Teronpi Amrita; Gayon Kaustav

Volume/Issue : Volume 9 - 2024, Issue 11 - November

Google Scholar : https://tinyurl.com/bdhjteev

Scribd : https://tinyurl.com/bduh48yh

DOI : https://doi.org/10.5281/zenodo.14414108

Abstract : The current study aims to regulate overabundance tomatoes by exploring the possibility of tomatoes' inherent antioxidants to safeguard lipid or fat-containing dietary items. Ghee loses quality as it gets heated because peroxide and free fatty acids are formed, among other things. Consequently, it is imperative to investigate methods and strategies to halt or reduce ghee's degradation. The purpose of the current investigation was to ascertain the solvent extraction yield of lycopene produced from tomatoes and the efficacy of lycopene in extending the shelf life of ghee relative to commonly used synthetic antioxidants. The experiment was conducted with six different concentrations of lycopene (0.01% w/w, 0.02% w/w, 0.05% w/w, 0.1% w/w, 0.25% w/w and 0.5% w/w) with ghee. The samples were cooled for two hours between each of the first three heat treatments (180 ± 50 C for 15 minutes). Percent inhibition values for radical scavenging activity (DPPH) gradually decreased as the number of heat treatments increased. The lowest decrease was observed with BHA (0.02%), and the maximum decrease was observed with control (unblended). However, up to 49 days, the performance of lycopene (0.02%) was also comparable to that of BHA. All heat treatments did not, however, cause BHA (0.02%) or lycopene (0.02%) to rise in peroxide levels in a same way. As the 49-day period went on, a similar pattern was also seen in the free fatty acid percentage. Being a naturally occurring antioxidant, lycopene (0.02%) may therefore be advised for the preservation of ghee following heat treatments.

Keywords : BHA; DPPH; Peroxide Value; Free Fatty Acid; Lycopene.

References :

1. Assous, M., Abdel, M. M, and Medany, G. M. (2014). Evaluation of red pigment extracted from purple carrots and its utilization as antioxidant and natural food colorants. Annals of Agricultural Science, 59: 1-7
2. Britton, G. (1995). Structure and properties of carotenoids in relation to function. FASEB Journal, 9: 1551–1558.
3. Blois, M. (1958). Antioxidant Determinations by the Use of a Stable Free Radical. Nature 181, 1199–1200. https://doi.org/10.1038/1811199a0
4. Choksi, P. and Joshi, V. (2007). A review on lycopene- extraction, purification, stability and applications. International Journal of Food Properties, 10: 289–298.
5. Di Mascio, P., Murphy, M. E. and Seis, H. (1991). Antioxidant defense systems: the role of carotenoids, tocopherols, and thiols. American Journal of Clinical Nutrition, 53: 194-200.
6. Fish, W. W., Veazie, P. P. and Collins, J. K. (2002). A Quantitative assay for lycopene that utilizes reduced volumes of organic solvents. Journal of Food Composition and Analysis, 15: 309–317.
7. Frankel, E. N. (1980). Lipid oxidation. Northern Regional Research Center, Agricultural Research, Science and Education Administration, U.S. Department of Agriculture. Progress in Lipid Research, PP 1-22.
8. FSSAI Manual of Methods of Analysis of Milk and Milk Products, 2015. (http://old.fssai.gov.in/Portals/0/Pdf/Draft_Manuals/MILK_AND_MILK_PRODUCTS.pdf). Ministry of Health and Family Welfare Government of India, New Delhi, pp 107-125.
9. FSSAI Manual of Methods of Analysis of Oils and Fats, 2015. (http://old.fssai.gov.in/Portals/0/Pdf/Draft_Manuals/OILS_AND_FAT.pdf). Ministry of Health and Family Welfare Government of India, New Delhi, pp 26-28.
10. Giese., J. (1996). Antioxidants: Tools for preventing lipid oxidation. Journal of Food Technology, 50:72.
11. Gulcin, I., Sat, I. G., Beydemir, S., and Kufrevioglu, O. I. (2004). Evaluation of the in vitro antioxidant properties of broccoli extracts (Brassica oleracea L.). Italian Journal of Food Sience, 16(1).
12. Kaur, H., and Perkins, J. (1991). The free radical chemistry of food additives. Journal of Free Radicals and Food Additives, 45(1):17-35.
13. Lilwani, S. and Nair, V. (2015). Extraction and isolation of lycopene form various natural sources. IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB), 01: 49-51.
14. Lin, C. and Chen, B. (2003). Determination of carotenoids in tomato juice by liquid chromatography. Journal of Chromatography A, 1012: 103–109.
15. Malviya, N. (2014). Isolation and quantification of lycopene from watermelon, tomato and papaya. Research Journal of Recent Sciences, 3: 68-70.
16. Mehta, B. (2006). Ragi (Eleusine Coracana L.) A natural antioxidant for Ghee (butter Oil). International Journal of Food Science and Technology, 41: 86-89.
17. Merai, M., Boghra, V. R. and Sharma, R. S. (2003). Extraction of antioxygenic principles from Tulsi leaves and their effects on oxidative stability of ghee. Journal of Food Science and Technology, 40: 52-57.
18. Molyneux, P. (2003). The use of stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology, 26(2): 211-219.
19. Patel, S., Shende, S., Arora, S., and Singh, A. K. (2013). An assessment of the antioxidant potential of coriander extract in ghee when stored at high temperature and during deep fat frying. International Journal of Dairy Technology, 66(2): 207-213.
20. Prieto, P., Pineda, M. and Aguilar, M. (1999). Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Journal of analytical biochemistry, 269: 337-341.
21. Schierle, J., Bretzel, W., Bühler, I., Faccin, N., Hess, D. and Steiner, K. (1997). Content of isomeric ratio of lycopene in food and human plasma. Journal of Food Chemistry. 59: 459–465.
22. Shoar, Z. D., Hardacre, A. K. and Brennan, C. S. (2010). The physico-chemical characteristics of extruded snacks enriched with tomato lycopene. Journal of Food Chemistry. 123: 1117-1122.
23. Sserun-jogi, M. L., Abrahamsen, R. K., and Narvhus, J. (1998). A review paper: current knowledge of ghee and related products. International Dairy Journal, 8(8), 677-688.
24. Trombly, H. H. and Porter, J. W. (1953). Additional carotenes and a colorless polyene of lycopersicon species and strains. Archives of Biochemistry and Biophysics. 43: 443-457.
25. Valverde, I., Periago, M., Provan, G.  and Chesson, A. (2002). Phenolic compounds, lycopene and antioxidant activity in commercial varieties of tomato. Journal of Food Science and Agriculture, 82: 323-330.
26. Velioglu, Y., Mazza, G., Gao, L., and Oomah, B. (1998). Antioxidant activity and total phenolics in selected vegetables and grain products. Journal of Agricultural Food Chemistry, 46: 4113-4117.
27. Wenli, Y., Yaping, Z., Zhen, X., Hui, J. and Dapu, W. (2001). The antioxidant properties of lycopene concentrated extracted from tomato paste. Journal of the American Oil Chemist Society, 78(7): 697-701.
28. Zeb, A. and Mehmood S. (2004). Carotenoids contents from various sources and their potential health applications.  Pakistan Journal of Nutrition, 3 (3): 199-204.
29. Zia-ur-Rehman, Salariya, Habib, F. (2003). Antioxidant activity of ginger extract in sunflower oil. Journal of Food Science and Agriculture, 83:624-629.