The study aimed to minimize the antibacterial agents prescription and hence bacterial resistance among health facilities, as best as. This goal could be achieved through tackling doctors’ awareness and attitudes towards the resistance issues. One more advantage is to enhance the practice of the doctors, making them always confident to prescribe antibiotics with considering the avoidance of unnecessary prescriptions.  Methodology: The study was conducted by distributing questionnaires to 75 doctors working at 15 different primary health centers. Knowledge and attitude were assessed through closed-ended statements. Knowledge has been assessed by reviewing the responses toward statements including the definition of irrational use of antibiotics, supportive laboratories investigations should be requested, safe antibiotics during pregnancy, antibiotics that can damage the liver and or kidney, and antibiotics-drug interactions. In regards to attitude, health professionals have been asked about several domains like the severity of the problem, the role of doctors in the irrational use of antibiotics, and barriers between doctors and applying the rational use of antibiotics concept. We gave scores for every statement choice, 2 points for the correct answer, 1 for the answer ‘I don’t know, and 0 for the incorrect answer. Total marks of each questionnaire collected, afterward, knowledge evaluated as good, moderate, or poor. Similarly, the attitude assessed after points collection as positive, neutral, or negative.  Results: The number of years spent in medical practice for the health professionals who participated in this study has been estimated. 92% have less than five years of medical experience, 6.7% have been practiced for 5-10 years, while 1.3% have been practiced for ten years. 33% of the total number of health professionals had 16-20 patients per day during the duration of the study. After marking and analyzing knowledge statements, results showed that 65% of doctors had poor knowledge, 31% got the moderate assessment, while 4% were good. For the attitude, the majority showed a neutral attitude (42%), doctors with a good attitude were 29%, while doctors with a poor attitude were 29%.  Conclusion: The knowledge about antibiotics of health professionals in the Khartoum locality, Sudan, has shown to be poor. Volume 6, Issue 10, October – 2021 International Journal of Innovative Science and Research Technology ISSN No:-2456-2165 IJISRT21OCT207 www.ijisrt.com 245 Attitude towards the concept of rational prescription of antibiotics has shown to be neutral.

Keywords : Antibiotics; Attitude; Knowledge; Khartoum; Sudan.