Isolation, Purification and Analysis of Pancreatic Lipase from ‘Gallus gallus domesticus’

Authors : Sandhya.B; Nagamani.T.S

Volume/Issue : Volume 5 - 2020, Issue 7 - July

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DOI : 10.38124/IJISRT20JUL338

This article discusses the isolation of pancreatic lipase enzyme from the pancreas of Gallus gallus domesticus. Whereas lipase catalyses the hydrolysis and the synthesis of esters formed from glycerol and long-chain fatty acids. Lipases occur widely in nature, it involves applications like organic syntheses, hydrolysis of fats, oils, modification of fats, flavor enhancement in food processing, detergent industries, pharmaceutical industries, chemical analyses, and biodiesel production. Pancreatic lipase was purified to the homogeneity by 70% saturated Ammonium sulphate further, it was dialysate using the dialysis membrane and then gel filtration chromatography was carried out by Sephadex G-75 and DEAE cellulose. The molecular weight of purified lipase sample was determined by SDSPAGE, it was found to be 98KDa. The lipase was active in the pH range of 5-10 with an optimum pH of 6.0. The optimum temperature for the hydrolysis of olive oil was 37ºC in the range of 25ºC - 50ºC.

Keywords : Gallus gallus domesticus, Lipases, Enzyme kinetics, Column chromatography, SDS-PAGE


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