Seven Naphthalene degrading bacteria were isolated from contaminated soil by enrichment medium utilize naphthalene as source of carbon and energy. Strains NS1A and MM3 grew best on enriched media were selected for biodegradation. The isolates were morphological and biochemically characterized as NS1A (Staphylococcus aureus) and MM3 (Pseudomonas fluorescens). Degradation of naphthalene efficacy of Staphylococcus aureus and Pseudomonas fluorescens is 63.7% and 50.17 % respectively after seven days incubation. The optimum pH and temperature for growth of isolates is 7.00 and 370C on 15-20% salt concentration. This potency of naphthalene degradation will increase the possible action to develop role model for hydrocarbon pollutant degradation from our surrounding environments.