Authors :
Shler Ali Khorsheed
Volume/Issue :
Volume 9 - 2024, Issue 2 - February
Google Scholar :
http://tinyurl.com/3duz2bys
Scribd :
http://tinyurl.com/4xd5e6u6
DOI :
https://doi.org/10.5281/zenodo.10721727
Abstract :
This study focuses on the isolation and
molecular quantification of bacteriostatic genes in
pathogenic bacteria prevalent in the Republican
Hospital in Kirkuk. Spore formation plays a pivotal role
in the virulence and persistence of pathogenic bacteria,
making it essential to understand the presence and
diversity of spore-forming genes in the hospital
environment. Using polymerase chain reaction (PCR)
technology, we aimed to develop a targeted and effective
method to detect bacteriostatic genes within bacterial
isolates obtained from clinical samples from 200
patients of different ages and genders at the Republican
Hospital. Bacterial isolates were collected from various
clinical sources using standard microbiological
protocols, and their identification was confirmed
through traditional microbiological methods. Bacillus
subtilis, Clostridium perfringens, Pseudomonas
aeruginosa Escherichia coli, Streptomyces aureus.
Enterobacter cloacae were isolated from 50% of
patients. Genomic DNA extraction was performed, and
PCR primers were designed to specifically amplify the
spore-forming gene region. The resulting PCR products
were visualized using gel electrophoresis to confirm the
presence of target genes. The result was the isolation of
the .spo0A gene. The study not only aimed to determine
the prevalence of spore-forming genes in pathogenic
bacteria, but also explored potential associations with
clinical outcomes and antibiotic resistance profiles. In
addition, bioinformatics tools were used to analyze
genetic evolution, highlighting the genetic diversity and
evolutionary aspects of bacteriostatic genes among
isolates. This research, conducted at the Republican
Hospital in Kirkuk, provides valuable insights into the
molecular characteristics of pathogenic bacteria in the
healthcare setting. The findings contribute to our
understanding of microbial dynamics within hospitals
and may inform infection control strategies.
Furthermore, the PCR-based approach provides a
rapid and sensitive diagnostic tool to detect spore-
forming genes, facilitating targeted interventions to
mitigate the impact of spore-forming bacteria on patient
health.
Keywords :
Polymerase Chain Reaction Technique (PCR)- Spore-Forming Bacteria – Genome- Spo0a Gene.
This study focuses on the isolation and
molecular quantification of bacteriostatic genes in
pathogenic bacteria prevalent in the Republican
Hospital in Kirkuk. Spore formation plays a pivotal role
in the virulence and persistence of pathogenic bacteria,
making it essential to understand the presence and
diversity of spore-forming genes in the hospital
environment. Using polymerase chain reaction (PCR)
technology, we aimed to develop a targeted and effective
method to detect bacteriostatic genes within bacterial
isolates obtained from clinical samples from 200
patients of different ages and genders at the Republican
Hospital. Bacterial isolates were collected from various
clinical sources using standard microbiological
protocols, and their identification was confirmed
through traditional microbiological methods. Bacillus
subtilis, Clostridium perfringens, Pseudomonas
aeruginosa Escherichia coli, Streptomyces aureus.
Enterobacter cloacae were isolated from 50% of
patients. Genomic DNA extraction was performed, and
PCR primers were designed to specifically amplify the
spore-forming gene region. The resulting PCR products
were visualized using gel electrophoresis to confirm the
presence of target genes. The result was the isolation of
the .spo0A gene. The study not only aimed to determine
the prevalence of spore-forming genes in pathogenic
bacteria, but also explored potential associations with
clinical outcomes and antibiotic resistance profiles. In
addition, bioinformatics tools were used to analyze
genetic evolution, highlighting the genetic diversity and
evolutionary aspects of bacteriostatic genes among
isolates. This research, conducted at the Republican
Hospital in Kirkuk, provides valuable insights into the
molecular characteristics of pathogenic bacteria in the
healthcare setting. The findings contribute to our
understanding of microbial dynamics within hospitals
and may inform infection control strategies.
Furthermore, the PCR-based approach provides a
rapid and sensitive diagnostic tool to detect spore-
forming genes, facilitating targeted interventions to
mitigate the impact of spore-forming bacteria on patient
health.
Keywords :
Polymerase Chain Reaction Technique (PCR)- Spore-Forming Bacteria – Genome- Spo0a Gene.
