Investigation of Anti-Microbial Activity of Juglans Regia L. against P. Aeruginosa and B. Subtilis


Authors : Aasif Manzoor Bhat; Mohd Altaf Khan; Rashida Qureshi; Mohd Yaseen

Volume/Issue : Volume 10 - 2025, Issue 6 - June


Google Scholar : https://tinyurl.com/nhc4crv4

DOI : https://doi.org/10.38124/ijisrt/25jun113

Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.


Abstract : Antibacterial activities of methanolic extract and ethyl acetate extract of Juglans regia . fruit husk cultivated in Kashmir were probed. Hot extraction process with methanol and ethyl acetate was carried out by employing Soxhlet apparatus method . The antimicrobial activity was calculated employing agar diffusion method. The extracts of fruit husk manifested antimicrobial activity against Pseudomonas aeruginosa as well as Bacillus subtilis along with minimum inhibitory concentration (MIC) of 100 μg/mL for Juglans regia (EA) extract against Pseudomonas aeruginosa,50 μg/mL for Juglans regia (MeOH) extract against Pseudomonas aeruginosa, 100 μg/mL for Juglans regia (EA) extract against Bacillus subtilis and 50 μg/mL for Juglans regia (MeOH) extract against Bacillus subtilis. The outcome established that the methanolic extract of Juglans regia fruit husk display antibacterial property against Pseudomonas aeruginosa and Bacillus subtilis.

Keywords : Juglans Regia Husk; Methanol Based Extract; Microbicidal Activity; Minimum Inhibitory Concentration.

References :

  1. Sharma P, Ravikumar G, Kalaiselvi M, Gomathi D, Uma C. In vitro antibacterial and free radical scavenging activity of green hull of Juglans regia. Journal of pharmaceutical analysis. 2013 Aug 1;3(4):298-302.
  2. Firenzuoli, F.; Gori, L. Evid Based Complement Alternat Med. 2007, 4, 37-40.
  3. Girzu M, Carnat A, Privat AM, Fialip J, Carnat AP, Lamaison JL. Sedative effect of walnut leaf extract and juglone, an isolated constituent. Pharmaceutical biology. 1998 Jan 1;36(4):280-6
  4. Tsao R. Chemistry and biochemistry of dietary polyphenols. Nutrients. 2010 Dec 10;2(12):1231-46
  5. Shojaii, A.; Dabaghian, F. H.; Goushegir, A.; Fard, M. A. Acta Med Iran. 2011, 49, 637-42. .
  6. Hu Q, Liu J, Li J, Liu H, Dong N, Geng YY, et al. Phenolic composition and nutritional attributes of diaphragma juglandis fructus and shell of walnut (Juglans regia L.). Food Sci Biotechnol 2020;29:187-9
  7. Jahanban-Esfahlan A, Ostadrahimi A, Tabibiazar M, Amarowicz R. A comparative review on the extraction, antioxidant content and antioxidant potential of different parts of walnut (Juglans regia L.) fruit and tree. Molecules 2019;24:2133.
  8. Bahmani, M.; Rafieian-Kopaei, M.; Hassanzadazar, H.; Saki, K.; Karamati, S. A.; Delfan, B. Asian Pacific journal of tropical medicine. 2014, 7, S29-S33.
  9. Li H, Horke S, Förstermann U. Oxidative stress in vascular disease and its pharmacological prevention. Trends Pharmacol Sci 2013;34:313-9.
  10. Kavak DD, Altiok E, Bayraktar O, Ülkü S. Pistacia terebinthus extract: as a potential antioxidant, antimicrobial and possible β-glucuronidase inhibitor. J Mol Catal B Enzym 2010;64:167-71.
  11. Acquaviva R, D’Angeli F, Malfa GA, Ronsisvalle S, Garozzo A, Stivala A, et al. Antibacterial and anti-biofilm activities of walnut pellicle extract (Juglans regia L.) against coagulase-negative staphylococci. Nat Prod Res 2021;35:2076-81.
  12. Chopra, R.; Nayar, S.; Chopra, I. New Delhi. 1986, 2-79.
  13. Prior RL, Cao G. In vivo total antioxidant capacity: comparison of different analytical methods. Free Radic Biol Med 1999;27:1173-81.
  14. Kusano C, Ferrari B. Total antioxidant capacity: a biomarker in biomedical and nutritional studies. J Cell Mol Biol 2008;7:1- 15.
  15. Chibane LB, Degraeve P, Ferhout H, Bouajila J, Oulahal N. Plant antimicrobial polyphenols as potential natural food preservatives. J Sci Food Agric 2019;99:1457-74.

Antibacterial activities of methanolic extract and ethyl acetate extract of Juglans regia . fruit husk cultivated in Kashmir were probed. Hot extraction process with methanol and ethyl acetate was carried out by employing Soxhlet apparatus method . The antimicrobial activity was calculated employing agar diffusion method. The extracts of fruit husk manifested antimicrobial activity against Pseudomonas aeruginosa as well as Bacillus subtilis along with minimum inhibitory concentration (MIC) of 100 μg/mL for Juglans regia (EA) extract against Pseudomonas aeruginosa,50 μg/mL for Juglans regia (MeOH) extract against Pseudomonas aeruginosa, 100 μg/mL for Juglans regia (EA) extract against Bacillus subtilis and 50 μg/mL for Juglans regia (MeOH) extract against Bacillus subtilis. The outcome established that the methanolic extract of Juglans regia fruit husk display antibacterial property against Pseudomonas aeruginosa and Bacillus subtilis.

Keywords : Juglans Regia Husk; Methanol Based Extract; Microbicidal Activity; Minimum Inhibitory Concentration.

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