Authors :
Danladi, G. H; Muhammad, S; Aliero, A. A; Isah, A. D; Gupta, A
Volume/Issue :
Volume 6 - 2021, Issue 12 - December
Google Scholar :
http://bitly.ws/gu88
Scribd :
https://bit.ly/3fd5c0u
Abstract :
Influence on germination of Dansalka and
Bahaushe millet (Pennisetum glaucum L. Br.) varieties by
biofertilizers produced from Azotobacter vinelandi and
Rhizobium phaseoli along with bacterial control abilities
of millet grains extract were studied using laboratory
facilities at Usmanu Danfodiyo University, Sokoto and
Center for Microbiology and Biotechnology, Bhopal.
Biofertilizers were produced from both microorganisms
using upstream and downstream fermentation method,
with Ashby and yeast extract mannitol broths as media.
Three working concentrations of 10, 15 and 20mg/ml
were obtained from each biofertilizer. Standard methods
were adopted for the germination studies, treatments
were replicated three times. Millet extracts of 60:40 water
and alcohol, were tested for in-vitro bacterial control by
plate disc method. Second day after sowing revealed no
germination from controls, but biofertilizer treatments
recorded highest germination rate from 20ml/l treatments
resulting in 63% for Dansalka in Azotobacter, 57% for
Dansalka and Bahaushe in Rhizobium and 53% for
Bahaushe in Rhizobium. The 15ml/l treatments followed
with 37% Bahaushe in Rhizobium and 33% for other
millets in both fertilizers. Full (100%) germination was in
20ml/l from 3rd day and by 4th day, all 20ml/l and 15ml/l
treated millets fully germinated. Dansalka in 10ml/l
Azotobacter germinated 100% on day 4, while the control
had 87% as final germination. Highest extract inhibition
was 15.33mm against A. vinelandi followed by 14.33mm
against R. phaseoli by 400mg/l. The lowest inhibition was
3.67mm on Xanthomonas axonopodis and X. campestris by
300mg/l. The 200mg/l showed no inhibition. Both
fertilizers positively influenced millet germination while
extract at highest concentration had mild inhibition of the
microorganisms. Therefore, Azotobacter and Rhizobium
biofertilizers are recommended especially for organic
millet cultivation
Keywords :
Millet Microorganisms Biofertilizers Germination Inhibition
Influence on germination of Dansalka and
Bahaushe millet (Pennisetum glaucum L. Br.) varieties by
biofertilizers produced from Azotobacter vinelandi and
Rhizobium phaseoli along with bacterial control abilities
of millet grains extract were studied using laboratory
facilities at Usmanu Danfodiyo University, Sokoto and
Center for Microbiology and Biotechnology, Bhopal.
Biofertilizers were produced from both microorganisms
using upstream and downstream fermentation method,
with Ashby and yeast extract mannitol broths as media.
Three working concentrations of 10, 15 and 20mg/ml
were obtained from each biofertilizer. Standard methods
were adopted for the germination studies, treatments
were replicated three times. Millet extracts of 60:40 water
and alcohol, were tested for in-vitro bacterial control by
plate disc method. Second day after sowing revealed no
germination from controls, but biofertilizer treatments
recorded highest germination rate from 20ml/l treatments
resulting in 63% for Dansalka in Azotobacter, 57% for
Dansalka and Bahaushe in Rhizobium and 53% for
Bahaushe in Rhizobium. The 15ml/l treatments followed
with 37% Bahaushe in Rhizobium and 33% for other
millets in both fertilizers. Full (100%) germination was in
20ml/l from 3rd day and by 4th day, all 20ml/l and 15ml/l
treated millets fully germinated. Dansalka in 10ml/l
Azotobacter germinated 100% on day 4, while the control
had 87% as final germination. Highest extract inhibition
was 15.33mm against A. vinelandi followed by 14.33mm
against R. phaseoli by 400mg/l. The lowest inhibition was
3.67mm on Xanthomonas axonopodis and X. campestris by
300mg/l. The 200mg/l showed no inhibition. Both
fertilizers positively influenced millet germination while
extract at highest concentration had mild inhibition of the
microorganisms. Therefore, Azotobacter and Rhizobium
biofertilizers are recommended especially for organic
millet cultivation
Keywords :
Millet Microorganisms Biofertilizers Germination Inhibition
