The present paper deals with the callus was raised from the leaf explant of jasminum sambac var. duplex voigt. The callus was raised on MS media supplemented with 3% sucrose with different concentration 2,4-D and BAP. A maximum callus was obtained at concentration of 2mg/lit maintained for 9 weeks for the growth. This callus was subjected to the chemical mutagen ( sodium azide). The effect of sodium azide at 3mm concentration the callus growth was enhance to 1.9 cm.
Jasminum Sambac. Var Duplex Voigt, Sodium Azide.