Authors : Kaye Stewarth M. Camacho; Shekinah Bliss C. Dote; Justine Deardrie N. Auxtero; Ruth Sophia A. Iglupas

Volume/Issue : Volume 11 - 2026, Issue 5 - May

Google Scholar : https://tinyurl.com/4wfskb3w

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DOI : https://doi.org/10.38124/ijisrt/26May264

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Abstract : This study aimed to evaluate the antibacterial activity of Beetroot (Beta vulgaris) leaf extract against Escherichia coli. Leaves were collected from Lantapan, Bukidnon, and extracted using 70% ethanol through rotary evaporation. The antibacterial activity of the extract was assessed at different concentrations (100%, 75%, 50%, and 25%) using the Kirby– Bauer disc diffusion method. Distilled water served as the negative control, while chloramphenicol was used as the positive control. Qualitative phytochemical screening revealed the presence of flavonoids, saponins, phenols, tannins, alkaloids, terpenoids, and steroids, indicating the existence of potentially bioactive compounds. However, results demonstrated that all tested concentrations of the beetroot leaf extract exhibited no antibacterial activity against E. coli, as evidenced by the absence of zones of inhibition comparable to the negative control and significantly lower than the positive control. These findings suggest that, under the conditions employed in this study, Beta vulgaris leaf extract does not exhibit effective antibacterial properties against E. coli. Nevertheless, the presence of phytochemicals indicates the potential for further investigation using alternative extraction methods or applications.

Keywords : Beta Vulgaris, Escherichia Coli, Distilled Water, Chloramphenicol, Flavonoids, Tannins, Saponins, Phenols, Steroids, Alkaloids, Terpenoids, Rotary Evaporator, Antibacterial Activity.

References :

1. Abdelkader, H., Ismail, S., & El-Hossary, E. (2018). Evaluation of antimicrobial activity of plant extracts using disc diffusion method. Journal of Microbiological Methods, 147, 55–60.
2. Andrews, J. M. (2001). Determination of minimum inhibitory concentrations. Journal of Antimicrobial Chemotherapy, 48(S1), 5–16.
3. Altavas, P. J. D., Abaya, A. R. G., Abella, R. V. T. D., Acosta, D. L. A., Aguilar, A. C., Aguinaldo, C. A. V., ... & Maramba-Lazarte, C. C. (2024). Antimicrobial Activity of Ardisia serrata (Cavs.) Pers. Ethanolic and Aqueous Leaf Extract on the Growth and Biofilm Formation of Selected Bacterial Isolates. Acta Medica Philippina, 58(18), 91.
4. Azwanida, N. N. (2015). A review on the extraction methods use in medicinal plants. International Journal of Basic & Clinical Pharmacology, 4(3), 491–496.
5. Bhargav, A. (2016). Evaluation of antimicrobial activity using agar disc diffusion method. International Journal of Current Microbiology and Applied Sciences, 5(4), 123–130.
6. Bjarnadottir, A. (2019). Beetroot (Beta vulgaris): Nutrition facts and health benefits. Healthline. (Replace with journal if required, but acceptable in some IJISRT papers)
7. Clinical and Laboratory Standards Institute (CLSI). (2020). Performance standards for antimicrobial susceptibility testing(30th ed.). CLSI supplement M100.
8. Galindo-Méndez, M. (2020). Escherichia coli infections and clinical implications. Infectious Disease Reports, 12(2), 45–52.
9. Jung, B., Hoilat, G. J., & Kamat, D. (2022). Gram-negative bacteria: Escherichia coli. StatPearls Publishing.
10. Kairupan, T. S., et al. (2019). Phytochemical screening and antioxidant activity of Beta vulgaris leaves. Journal of Applied Pharmaceutical Science, 9(3), 85–90.
11. Martinson, J. N. V., & Walk, S. T. (2020). Escherichia coli: Ecology and role in the gut microbiome. Microbiology Spectrum, 8(4), 1–15.
12. Martin, K. W., & Ernst, E. (2003). Herbal medicines for treatment of bacterial infections: A review. Journal of Antimicrobial Chemotherapy, 51(2), 241–246.
13. Mounyr Balouiri, M., Sadiki, M., & Ibnsouda, S. K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, 6(2), 71–79.
14. Mueller, L., et al. (2023). Pathogenesis and antimicrobial resistance of Escherichia coli. Frontiers in Microbiology, 14, 118–130.
15. Olivi, G., et al. (2014). Antimicrobial activity of plant extracts and controls in microbiological assays. Journal of Clinical Microbiology, 52(4), 120–125.
16. Pangeni, R., et al. (2021). Evaluation of antibacterial activity of plant extracts using Kirby-Bauer method. BMC Research Notes, 14, 266.
17. Poirel, L., Madec, J. Y., Lupo, A., Schink, A. K., Kieffer, N., Nordmann, P., & Schwarz, S. (2018). Antimicrobial resistance in Escherichia coli. Microbiology Spectrum, 6(4), 1–27.
18. Rutala, W. A., Weber, D. J., & Healthcare Infection Control Practices Advisory Committee. (2019). Guideline for disinfection and sterilization in healthcare facilities. Centers for Disease Control and Prevention (CDC).
19. Salam, M. A., et al. (2023). Chloramphenicol and its antibacterial efficacy against Gram-negative bacteria. Antibiotics, 12(5), 789.
20. Singh, P., et al. (2017). Antibacterial activity of plant extracts: A comprehensive review. International Journal of Pharmaceutical Sciences Review and Research, 44(1), 45–50.
21. Thakur, M., et al. (2020). Phytochemicals: Potential therapeutic agents. Journal of Pharmacognosy and Phytochemistry, 9(1), 245–250.
22. Wiegand, I., Hilpert, K., & Hancock, R. E. (2008). Agar and broth dilution methods to determine MIC. Nature Protocols, 3(2), 163–175.