The present paper deals with invitro study of anti-fungal activity in Eucalyptus globules labill in acetone and chloroform extract. The extraction was made by Soxhlet’s apparatus. The anti fungal properties were determined by poison food technique method using test fungi such as Curvularia lunata, Drechslera avenaceum, Fusarium oxysporium, Aspergillus niger and Trichoderma viridi. These test fungi were found to be sensitive to extract as compared to control. A least zone were recorded against Fusarium oxysporium both acetone and chloroform extract. Proves to be possess good fungicidal properties followed by against Trichoderma viridi and Curvularia lunata.

Keywords : Curvularia Lunata, Drechslera Avenceum, Fusarium Oxysporium, Aspergillu Sniger, Trichoderma Viridi, Soxhlets Apparatus.