Authors : Azhari; Ismail; Rastina

Volume/Issue : Volume 9 - 2024, Issue 5 - May

Google Scholar : https://tinyurl.com/3ya3bmkm

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DOI : https://doi.org/10.38124/ijisrt/IJISRT24MAY2495

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Abstract : The use of feed ingredients has not been addressed until now, in the sense that competition between food and feed is still continuing, especially protein source feed, thus causing a dilemma for farmers.Therefore, in connection with the steps that will be taken in efforts to health livestock and fish farming that will be carried out, efforts are needed in preventing bacterial-based diseases. The purpose of this study was to determine the diversity of all bacteria, both pathogenic and non-pathogenic bacteria found in the magot digestive system using 16S rRNA gene analysis. The results of this study will be a scientific reference for the development of maggot asasafe, effective, and efficient alternative to animal feed.Based on the results of the studies obtained, it can be concluded that the bacteria contained in the digestion of maggots show a diversity of pathogenic and non-pathogenic bacteria, with the form of bacil and cocobacil. Inaddition, DNA extraction and ba cterial identification have also been carried out using the PCR method. The results of the sequencing of the seven bacterial isolates of magot digestive origin showed gooddendograms and nucleotide sequences, so the next stage was bioinformatics analysis and phylogenetic construction, to identify species and their kinship relationships between species.

Keywords : Maggots, PCR, Larvae, Insecta, Animal Feed.

References :

1. Afkar,K.,Masrufah,A.,Fawaid,A.S.,Alvarizi,D.W.,Khoiriyah,L.,Khoiriya h,M., Kafi, M.A., Faradilla, R.S., Amsah, R., Hidayah, N.N., Salsabella, A., Nazwa, D.A.R., Fadila, S.N., Sari, U.E.K., Naim, F.I., Itsnaini, S.N.R. dan Ramadhan, M.N. (2020). Budidaya maggot BSF (Black Soldier Fly) sebagai pakan alternatif ikan lele (Clarias batracus) di desa candipari, sidoarjo pada program holistic pembinaan dan pemberdayaan dea (PHP2D). Journal of Science and Social Development, 3:10-16.
2. Amandanisa, A.dan Suryadarma, P.(2020). Kajiannutrisidanbudidayamagg ot (Hermentia illuciens L.) sebagai alternatif pakan ikan diRT 02 Desa Purwasari, Kecamatan Dramaga, Kabupaten Bogor. Jurnal PusatInovasi Masyarakat, 2(5):796‒804.
3. Bosch, G., Zhang, S., Dennis, G.A.B.O. dan Wouter, H.H. (2014). Protein quality of insects as potential ingredients for dog and cat foods. J Nutr Sci, 3:1-4.
4. Erickson, M.C., Islam, M., Sheppard, C., Liao, J.danDoyle, M.P. (2004). Reductionof Escherichia coli O157:H7 and Salmonella enterica serovar Enteritidis in chicken manurebylarvaeofthe Black Soldier Fly. J Food Prot. 67:685-690.
5. Fahmi, M. R., Hem, S. dan Subamia, I. W. (2009). Potensi maggot untuk peningkatan pertumbuhan dan status kesehatan ikan. J. Ris. Akuakultur, 4(2):221-232
6. Fahmi, M.R.(2010). Manajemen pengembangan maggot menuju kawasanpakan mina mandiri. Dalam: Prosiding Forum Inovasi Fatmasari, L. (2017). Tingkat densitas populasi, bobot, dan panjang maggot (Hermetia illucens) padamedia yang berbeda. Skripsi. Universitas Islam Negeri RadenIntan, Lampung.
7. Fatmasari, L. (2017). Tingkat densitas populasi, bobot, dan panjang maggot (Hermetia illucens) padamedia yang berbeda. Skripsi. Universitas Islam Negeri RadenIntan, Lampung.
8. Gulaydin,O.,Ekin,I.H.,Ozturk,C.,Ilhan,Z.danOrgun,E.(2019).comparison ofsome bacterial identification methods. Turkish Journal of Veterinary Research, 3 (1): 9- 12.
9. Indarmawan. (2014). Hewan Avertebrata Sebagai Pakan Ikan Lele.
10. Skripsi. Fakultas Biologi Universitas Jenderal Soedirman, Purwokerto.
11. Kresnawaty, I., Wahyu, R. dan Sasongko, A. 2018. Aktivitas amilase bakteri amilolitik asal larva black soldier fly (Hermetia illucens). Menara Perkebunan. 87(2) : 140- 146.
12. Pamaya, D., Muchlissin, S. I., Maharani, E.T.W., Darmawati, S. dan Ethica, S.N. 2018. Isolase bakteri penghasil enzim protease bacillus amiloliquefa irod2 pada         oncom merahpascafermentasi48jam. Seminar Nasional Edusaintek.Semarang,Indon esia.
13. Slabbinck, B., Waegeman, W., Dawyndt, P., De Vos, P., De Baets, B., 2010. From learning taxonomies to phylogenetic learning: integration of 16S rRNA gene data into FAME-based bacterial classification. BMC Bioinf. 11, 69.

14.Srinivasan,R.,Karaoz,U.,Volegova,M.,etal.,2015.Useof16SrRNAgenefor identificationofabroadrangeofclinicallyrelevantbacterialpathogens.PLoS ONE 10.e0117617.