Authors :
Sabrine Y.Yasenge; HonoréS. Muhindo; ClerisseM. Casinga; DidyO. Onausthu; GodefroidT.K. Monde
Volume/Issue :
Volume 7 - 2022, Issue 4 - April
Google Scholar :
https://bit.ly/3IIfn9N
Scribd :
https://bit.ly/3lhXGV3
DOI :
https://doi.org/10.5281/zenodo.6555343
Abstract :
The objective of the present study was to
identify the viral strains associated with cassava brown
streak disease, to determine the cassava cultivars used,
their status (local or improved), and the age of cassava
crops in the field, and to study its distribution in the
Province of North Kivu. The modified Cetyltrimethyl
ammonium bromide method was used to extract RNA
from samples of collected cassava leaves. A pair of
primers was used in diagnosis to confirm the presence of
viral strains on the samples. Cassava cultivars followed
by their status and age in cultivation were determined.
Georeferenced survey data and molecular analysis data
were integrated into molecular incidence and field
incidence maps. Our results show that the cassava brown
streak disease is spread in all the territories of North
Kivu, the most infected are the territories of Beni,
Masisi, and Rutshuru while the least infected are Lubero
and Nyiragongo. The improved cultivars are the most
cultivated in Beni and Lubero (5 improved against 4
local and 5 improved against 2 local respectively). The
average age of the crops is 6 months. Out of 380 samples
tested, 74 (19%) reacted positively to the Cassava brown
streak virus. Cassava brown streak disease is recognized
as a major constraint in the cassava fields of farmers in
North Kivu caused by Cassava brown streak virus and is
spread in all the territories surveyed. The cassava
cultivars used are improved and local with an average
age of 6 months.
Keywords :
Determination, cassava brown streak disease, Molecular incidence, Field incidence, North Kivu.
The objective of the present study was to
identify the viral strains associated with cassava brown
streak disease, to determine the cassava cultivars used,
their status (local or improved), and the age of cassava
crops in the field, and to study its distribution in the
Province of North Kivu. The modified Cetyltrimethyl
ammonium bromide method was used to extract RNA
from samples of collected cassava leaves. A pair of
primers was used in diagnosis to confirm the presence of
viral strains on the samples. Cassava cultivars followed
by their status and age in cultivation were determined.
Georeferenced survey data and molecular analysis data
were integrated into molecular incidence and field
incidence maps. Our results show that the cassava brown
streak disease is spread in all the territories of North
Kivu, the most infected are the territories of Beni,
Masisi, and Rutshuru while the least infected are Lubero
and Nyiragongo. The improved cultivars are the most
cultivated in Beni and Lubero (5 improved against 4
local and 5 improved against 2 local respectively). The
average age of the crops is 6 months. Out of 380 samples
tested, 74 (19%) reacted positively to the Cassava brown
streak virus. Cassava brown streak disease is recognized
as a major constraint in the cassava fields of farmers in
North Kivu caused by Cassava brown streak virus and is
spread in all the territories surveyed. The cassava
cultivars used are improved and local with an average
age of 6 months.
Keywords :
Determination, cassava brown streak disease, Molecular incidence, Field incidence, North Kivu.
