Authors :
Hargono Hargono; Aldi Asian Pandiangan; Ivander Wonderix Lumban Gaol; Kristinah Haryani; Hantoro Satriadi; Kevin Setiadi Seng
Volume/Issue :
Volume 8 - 2023, Issue 9 - September
Google Scholar :
https://bit.ly/3TmGbDi
Scribd :
https://tinyurl.com/8fwy5zuj
DOI :
https://doi.org/10.5281/zenodo.8409159
Abstract :
Bioethanol is an alternative fuel as a mixture
of conventional fuel. The production of bioethanol
involves the fermentation of biomass with live
microorganism cells. One of the raw materials that is
lacking in use but has great potential for bioethanol
production is durian seed flour. The production process
of bioethanol from durian seed flour includes enzymatic
hydrolysis using a mixture of alpha-amylase and
glucoamylase enzymes at concentrations of 100, 150, and
200 g/L of durian seed flour. Subsequently, the
fermentation process is carried out using Saccharomyces
cerevisiae yeast. This study aimed to investigate the
effects of adding metal ions Cu2+ and Fe3+ during the
processes of hydrolysis and fermentation. The research
methodology begins with the preparation of durian seed
flour. Furthermore, the durian seed flour is hydrolyzed
by adding metal ions such as Cu2+ and Fe3+, followed by
the analysis of glucose concentration. Higher substrate
concentrations lead to higher glucose concentrations.Before the fermentation stage, a
Saccharomyces cerevisiae starter culture is prepared.
Based on the experimental results, the addition of Cu2+
and Fe3+ metal ions has a positive effect as activators in
the enzymatic hydrolysis process.This aligns with the theory that the addition of
Fe3+ enhances yeast cell growth during the fermentation
process.
Keywords :
Bioethanol; Durian Seed Flour; Fermentation; Mineral Ion.
Bioethanol is an alternative fuel as a mixture
of conventional fuel. The production of bioethanol
involves the fermentation of biomass with live
microorganism cells. One of the raw materials that is
lacking in use but has great potential for bioethanol
production is durian seed flour. The production process
of bioethanol from durian seed flour includes enzymatic
hydrolysis using a mixture of alpha-amylase and
glucoamylase enzymes at concentrations of 100, 150, and
200 g/L of durian seed flour. Subsequently, the
fermentation process is carried out using Saccharomyces
cerevisiae yeast. This study aimed to investigate the
effects of adding metal ions Cu2+ and Fe3+ during the
processes of hydrolysis and fermentation. The research
methodology begins with the preparation of durian seed
flour. Furthermore, the durian seed flour is hydrolyzed
by adding metal ions such as Cu2+ and Fe3+, followed by
the analysis of glucose concentration. Higher substrate
concentrations lead to higher glucose concentrations.Before the fermentation stage, a
Saccharomyces cerevisiae starter culture is prepared.
Based on the experimental results, the addition of Cu2+
and Fe3+ metal ions has a positive effect as activators in
the enzymatic hydrolysis process.This aligns with the theory that the addition of
Fe3+ enhances yeast cell growth during the fermentation
process.
Keywords :
Bioethanol; Durian Seed Flour; Fermentation; Mineral Ion.
