Authors :
Kehinde Ibrahim Oluwatobi; Olatunji Oluwaseun Emmanuel; Awotuya Iyanu Oluwafemi; Adegoke Azeez
Volume/Issue :
Volume 7 - 2022, Issue 4 - April
Google Scholar :
https://bit.ly/3IIfn9N
Scribd :
https://bit.ly/3uN7H2c
DOI :
https://doi.org/10.5281/zenodo.6464836
Abstract :
The study obtained the crude extract of
Abelmoschus esculentus (Okra) fruits, isolated the
compounds from the extract and characterized the
structures of the isolated compounds. It also investigate
the histological effect of the crude extracts and its isolated
compounds on mice heart. These helps in providing
information and facts on the cardiovascular activities of
A. esculentus (Okra) fruit extract and its isolated
compounds on mice heart. The Abelmoschus esculentus
fruits were collected, identified, dehydrated (air dried),
and pulverized. The grinded plant material was macerate
with 100% ethanol for 72 hours. Separation of the
phytochemicals from the extract was achieved by
repeated open chromatographic procedures. The isolated
compounds were subjected to spectroscopic analysis such
as mass spectrometry (MS), ultraviolet (UV) and nuclear
magnetic resonance (NMR). The histological effects of the
crude extracts and its isolated compound was evaluated,
the animals were divided into digoxin (reference or
positive control), distilled water (negative control) and
treatment (administered the crude extract or isolated
compounds) groups. A dose of 1 mg/kg body weight of
digoxin and a dose of 10 mg/kg body weight of the crude
extract or isolated compounds were administered daily
intraperitonealy for ten days. The animals were sacrificed
on the eleventh day using diethyl ether as anaesthetic
agent with their heart harvested and preserved using 10
% formalin followed by the histological studies on the
isolated organ (heart). Three compounds from two
different classes of compounds were isolated and
elucidated from A. esculentus (two flavonoids and one
triterpene). Also demonstrated cardiovascular activities
of Abelmoschus esculentus extract and its isolated
compounds on mice heart. The flavonoid glycosides
(Quercetin glycoside and Quercetin diglycoside) present
in A. esculentus preserved the cardiac muscle
architecture similarly to digoxin the classical cardiac
glycoside except Ursene-3-O-β-D-Glucopyranoside which
shows dilatation of the cardiac muscle and some
degenerative nuclei
Keywords :
Isolation, Elucidation, Histological Effect, Cardiovascular Activities, Abelmoschus esculentus, Flavonoid, Triterpene
The study obtained the crude extract of
Abelmoschus esculentus (Okra) fruits, isolated the
compounds from the extract and characterized the
structures of the isolated compounds. It also investigate
the histological effect of the crude extracts and its isolated
compounds on mice heart. These helps in providing
information and facts on the cardiovascular activities of
A. esculentus (Okra) fruit extract and its isolated
compounds on mice heart. The Abelmoschus esculentus
fruits were collected, identified, dehydrated (air dried),
and pulverized. The grinded plant material was macerate
with 100% ethanol for 72 hours. Separation of the
phytochemicals from the extract was achieved by
repeated open chromatographic procedures. The isolated
compounds were subjected to spectroscopic analysis such
as mass spectrometry (MS), ultraviolet (UV) and nuclear
magnetic resonance (NMR). The histological effects of the
crude extracts and its isolated compound was evaluated,
the animals were divided into digoxin (reference or
positive control), distilled water (negative control) and
treatment (administered the crude extract or isolated
compounds) groups. A dose of 1 mg/kg body weight of
digoxin and a dose of 10 mg/kg body weight of the crude
extract or isolated compounds were administered daily
intraperitonealy for ten days. The animals were sacrificed
on the eleventh day using diethyl ether as anaesthetic
agent with their heart harvested and preserved using 10
% formalin followed by the histological studies on the
isolated organ (heart). Three compounds from two
different classes of compounds were isolated and
elucidated from A. esculentus (two flavonoids and one
triterpene). Also demonstrated cardiovascular activities
of Abelmoschus esculentus extract and its isolated
compounds on mice heart. The flavonoid glycosides
(Quercetin glycoside and Quercetin diglycoside) present
in A. esculentus preserved the cardiac muscle
architecture similarly to digoxin the classical cardiac
glycoside except Ursene-3-O-β-D-Glucopyranoside which
shows dilatation of the cardiac muscle and some
degenerative nuclei
Keywords :
Isolation, Elucidation, Histological Effect, Cardiovascular Activities, Abelmoschus esculentus, Flavonoid, Triterpene
