Authors :
Anjana Priyadarshani Kanathala; Srinivas Naik Kethavath; Prashanth Bollempally; Anjana Wahengbam; Prashant Singam; Sriya Reddy Patlolla
Volume/Issue :
Volume 9 - 2024, Issue 3 - March
Google Scholar :
https://tinyurl.com/35jhmu5b
Scribd :
https://tinyurl.com/bdee4ejj
DOI :
https://doi.org/10.38124/ijisrt/IJISRT24MAR1492
Abstract :
For most people across the planet, rice is also
considered as most beneficial crops in terms of fulfilling
every day's energy and nutritional needs. Worldwide
food safety is seriously threatened since severe
limitations on rice output caused by both abiotic and
biotic variables. Even though rice features much more
developed regeneration mechanism than other
agricultural crops, majority of desi cultivars continue to
stay as impervious to genetic alterations and
regeneration. Therefore, among preliminary steps in the
transgenic plant generation necessitates to improve
tissue culture procedures to produce viable plants via
embryogenic calli mediated regeneration. In this study,
we reporting that enhanced rate of plant regeneration
was observed from mature seed-derived embryogenic
calli of the indica rice cultivar MTU1010 following
partially desiccation procedure. We studied the effects of
several plant growth regulators on the efficiency of
inducing embryogenic callus and regeneration of whole
plantlets. The MS-CIM medium with 2.0 mg-1 2,4, D
showed the highest callus induction percentage.
Maximum rate of shoot regeneration (92%) was
observed from 48hr partially dehydrated calli, when
enriched with high cytokinin (2 mg/l kinetin) and lower
auxin (0.5 mg/l NAA) concentrations. Calli that had been
moderately dehydrated showed significantly greater
regeneration rates than undehydrated calli. Upon being
transferred onto a rooting medium, the well-rooted
plantlets were shifted to shaded glass house for
hardening. Hence, this approach is rather simple to
apply and can be employed in genetic modification
studies alongside other biotechnological endeavours.
Keywords :
Rice, MTU1010, Desiccation, Callus Induction, In vitro Regeneration.
For most people across the planet, rice is also
considered as most beneficial crops in terms of fulfilling
every day's energy and nutritional needs. Worldwide
food safety is seriously threatened since severe
limitations on rice output caused by both abiotic and
biotic variables. Even though rice features much more
developed regeneration mechanism than other
agricultural crops, majority of desi cultivars continue to
stay as impervious to genetic alterations and
regeneration. Therefore, among preliminary steps in the
transgenic plant generation necessitates to improve
tissue culture procedures to produce viable plants via
embryogenic calli mediated regeneration. In this study,
we reporting that enhanced rate of plant regeneration
was observed from mature seed-derived embryogenic
calli of the indica rice cultivar MTU1010 following
partially desiccation procedure. We studied the effects of
several plant growth regulators on the efficiency of
inducing embryogenic callus and regeneration of whole
plantlets. The MS-CIM medium with 2.0 mg-1 2,4, D
showed the highest callus induction percentage.
Maximum rate of shoot regeneration (92%) was
observed from 48hr partially dehydrated calli, when
enriched with high cytokinin (2 mg/l kinetin) and lower
auxin (0.5 mg/l NAA) concentrations. Calli that had been
moderately dehydrated showed significantly greater
regeneration rates than undehydrated calli. Upon being
transferred onto a rooting medium, the well-rooted
plantlets were shifted to shaded glass house for
hardening. Hence, this approach is rather simple to
apply and can be employed in genetic modification
studies alongside other biotechnological endeavours.
Keywords :
Rice, MTU1010, Desiccation, Callus Induction, In vitro Regeneration.
