Authors : Emakulata. E. Ndolimana; Dr. Alex Shayo

Volume/Issue : Volume 10 - 2025, Issue 5 - May

Google Scholar : https://tinyurl.com/mwbh4tp2

DOI : https://doi.org/10.38124/ijisrt/25may932

Note : A published paper may take 4-5 working days from the publication date to appear in PlumX Metrics, Semantic Scholar, and ResearchGate.

Abstract : Carbapenem-resistant Klebsiella pneumoniae strains pose a significant threat to public health due to their ability to produce the blaKPC gene, conferring resistance to carbapenem antibiotics. Understanding the genomic diversity, evolutionary relationships and genetic complexity of the blaKPC variants is crucial for effective antimicrobial strategies. The study involved comprehensive genomic analysis of blaKPC gene sequences from 55 K. pneumoniae strains. The study involved indel haplotype diversity assessment, nucleotide diversity calculations and sequence conservation analysis. Additionally, the results were compared with larger dataset from a previous study. Indel haplotype diversity was Hd =0.879, nucleotide genetic variation was π = 0.0395 and the average number of differences in nucleotide was 37.446. Despite a smaller sample size, the study shows increased diversity of 8.9%. This study contributes valuable insights into blaKPC gene diversity, evolution and clinical relevance. Recommendations include tailored antibiotic selection, phylogenetic surveillance and global collaboration. Future studies should focus on functional characterization and clinical validation.

References :

1. Ashurst JV, D. A. (2023). Klebsiella Pneumonia. StatPearls [Internet] Treasure Island (FL) :StatPearls Publishsing. https://www.ncbi.nlm.nih.gov/books/NBK519004/
2. Becker Laura, Fuchs Stephan, Pfeifer Yvonne, Semmler Torsten, Eckmanns Tim, Korr Gerit, Sissolak Dagmar, Friedrichs Michael, Zill Edith, Tung Mei-Lin, Dohle Christian, Kaase Martin, Gatermann Sören, Rüssmann Holger, Steglich Matthias, Haller Sebastian, W. G. (2018). Whole Genome Sequence Analysis of CTX-M-15 Producing Klebsiella Isolates Allowed Dissecting a Polyclonal Outbreak Scenario. Frontiers in Microbiology, 9(1664-302X). https://doi.org/10.3389/fmicb.2018.00322
3. Ebomah, K. E., & Okoh, A. I. (2020). Detection of carbapenem-resistance genes in klebsiella species recovered from selected environmental niches in the Eastern Cape Province, South Africa. Antibiotics, 9(7), 1–12. https://doi.org/10.3390/antibiotics9070425
4. Forero-hurtado, D., Corredor-rozo, Z. L., M, R. A., Abril, D., Vanegas, N., In, G., Chambrone, L., & Escobar-p, J. (2023). Worldwide Dissemination of bla KPC Gene by Novel Mobilization Platforms in Pseudomonas aeruginosa : A Systematic Review.
5. In, H., Province, S., Chi, X., Hu, G., Xu, H., Li, X., Xiao, T., & Zhou, Y. (2022). Genomic Analysis Of A KPC-2-Producing Klebsiella Pneumoniae ST11 Outbreak From A Teaching Genomic Analysis Of A KPC-2-Producing Klebsiella Pneumoniae ST11 Outbreak From ATeaching Hospital In Shandong Province , China. https://doi.org/10.2147/IDR.S221788
6. Ma, L., & Liu, Y. (2022). The Genomic Characterization of KPC-Producing Klebsiella pneumoniae from the ICU of a Teaching Hospital in Shanghai , China. October 2021.
7. Mendez, M. G. (2020). Antimicrobial Resistance in Escherichia coli (L. Rodrigo (ed.)). https://www.intechopen.com/chapters/72615
8. Moyo, P., Moyo, E., Mangoya, D., Mhango, M., Mashe, T., Imran, M., & Dzinamarira, T. (2023). Prevention of antimicrobial resistance in sub-Saharan Africa: What has worked? What still needs to be done? Journal of Infection and Public Health.
9. Paterson, D. L. (2006). Resistance in gram-negative bacteria: Enterobacteriaceae. American Journal of Infection Control, Volume 34(Issue 5, Supplement), S20–S28. https://doi.org/10.1016/j.ajic.2006.05.238.%0A(https://www.sciencedirect.com/science/article/pii/S0196655306008534)
10. Rawat, D., & Nair, D. (2010). Extended-spectrum ß-lactamases in gram negative bacteria. Journal of Global Infectious Diseases, 2(3), 263. https://doi.org/10.4103/0974-777x.68531
11. Rozas,J., Ferrer-Mata,A.,Sanchez-DelBarrio,J.C, Guirao-Rico, S., Librado,P., Ramos-Onsins S.E, Sanchez-Gracia, A. (2017). DnaSP v6: DNA sequence polymorphism analysis of Large Dataset.
12. Shanmugam, P., Meenakshisundaram, J., & Jayaraman, P. (2013). bla KPC gene Detection in Clinical Isolates of Carbapenem Resistant Enterobacteriaceae in a Tertiary Care Hospital. 7(12), 2736–2738. https://doi.org/10.7860/JCDR/2013/7759.3747
13. Spadar, A., Perdigão, J., Campino, S., & Clark, T. G. (2023). Large-scale genomic analysis of global Klebsiella pneumoniae plasmids reveals multiple simultaneous clusters of carbapenem-resistant hypervirulent strains. Genome Medicine, 15(1), 3. https://doi.org/10.1186/s13073-023-01153-y
14. Tuhamize, B., Asiimwe, B. B., Kasaza, K., Sabiiti, W., Holden, M., & Bazira, J. (2023). Klebsiella pneumoniae carbapenamases in Escherichia coli isolated from humans and livestock in rural south-western Uganda. PLoS ONE, 18(7 July), 1–10. https://doi.org/10.1371/journal.pone.0288243
15. Uchil, R. R., Kohli, G. S., KateKhaye, V. M., & Swami, O. C. (2014). Strategies to combat antimicrobial resistance. Journal of Clinical and Diagnostic Research: JCDR, 8(7), ME01.